Patients with ABI < 0.9 showed a higher prevalence of carotid atherosclerosis. There was a negative correlation between ABI and IMT.

Background The human skin is an extremely sophisticated and evolved organ that covers the whole body. External agents or the patient’s own diseases can cause skin injuries that can challenge healthcare professionals and impose high social, economic and emotional costs. Objectives To evaluate the impact of topical nifedipine on skin wound healing, specifically on polymorphonuclear cells, vascular proliferation, and collagen. Methods We used three pigs, and created eight injuries in the dorsal region of each animal. We applied 1%, 10%, and 20% concentration nifedipine creams to four of the wounds in animals 1, 2, and 3 respectively and treated the other twelve wounds with saline solution 0.9% only. We analyzed the presence of polymorphonuclear cells, vascular proliferation, and collagen at six different times (days 1, 3, 7, 14, 21, and 28). Results The evaluation of polymorphonuclear levels showed mild cell activity at all times in the control group, while in the nifedipine groups, marked levels were more frequent at all times during the experiment. There was a 4.84-fold increase in the chance of marked vascular proliferation (p = 0.019) and, at the same time, a decrease in collagen formation (OR 0.02 / p = 0.005) in animal 3. Conclusions Topical NFD may have an impact on skin wound healing mechanisms. Our study showed that polymorphonuclear cells and vascular proliferation increased. We also demonstrated that collagen formation decreased. Therefore, topical NFD may have a positive impact on skin wound healing. Additional studies are needed to confirm our results.

Introduction: Skin wounds are a problem and many occur due to vascular diseases. There is a need for drugs (low cost) that can heal wounds. We aimed to evaluate if the use of nifedipine in wounds is associated with recruitment of polymorphonuclear cells higher than placebo. Hypothesis: Nifedipine stimulates the recruitment of polymorphonuclear in wounds. Methods: We performed 32 wounds in pigs and locally applied placebo or nifedipine (1%, 10% or 20%). Wounds were evaluated macro and microscopically at 6 different moments. A logistic longitudinal model of mixed effects was applied. For this purpose, the response to moderate or marked polymorphonuclear cells was considered as a dependend variable, with the comparison groups (placebo, nifedipine 1%, 10% and 20%) as the explanatory variable. Based on the placebo group, the OR was estimated with its respective confidence interval for the nifedipine groups at the different doses. Value of p <0.05 was considered significant. This study was approved by the animal research ethics committee. Results: The table below shows the proportion of polymorphonuclear cells among the comparison groups over the 6 analyzed moments. Conclusions: Wounds treated with nifedipine had higher recruitment of polymorphonuclear, regardless of the concentration used. This drug has potential for recruitment of this type of cell and may have a beneficial effect on healing.

Introduction: Loss of skin integrity can result in a wound. Wounds are associated with high cost and disability for patients. Peripheral arterial disease may be the cause of wounds that infect and lead to amputations. Our objective was to evaluate whether the use of nifedipine in wounds is associated with greater amounts of new vascular formations and collagen when compared to placebo. Hypothesis: The new vascular formation in wounds is greater in those treated with nifedipine than in those treated with placebo. Methods: We performed 32 wounds in pigs and locally applied placebo or nifedipine (1%, 10% or 20%). Wounds were evaluated macro and microscopically at 6 different moments. A logistic longitudinal model of mixed effects was applied. For this purpose, the response to moderate or marked polymorphonuclear cells was considered as a dependend variable, with the comparison groups (placebo, nifedipine 1%, 10% and 20%) as the explanatory variable. Based on the placebo group, the OR was estimated with its respective confidence interval for the nifedipine groups at the different doses. Value of p <0.05 was considered significant. This study was approved by the animal research ethics committee. Results: The figure below shows the comparison of new vascular formation and collagen between groups at six different times. Conclusions: The use of nifedipine in wounds was associated with greater vascular formation when compared to placebo. There were differences in the production of collagen between the groups evaluated (in the groups of nifedipine 10% and 20% less amount of collagen). Nifedipine may have a positive impact on the healing of skin wounds.