The purpose of this study was to evaluate the efficacy of some biological methods for removing toxins or reducing the toxic effect of aflatoxin. Initially, the genus Aspergillus spp was isolated from chronic lung infections, Sputum samples were collected from patients with chest and respiratory diseases who visited the consulting clinic, and the samples were subjected to direct microscopic examination and agricultural and molecular diagnosis using PCR technology. Aflatoxin B1 chemical detection also indicated the use of some poison detection methods, such as the ammonia vapour method, a kind-layer chromatic analysis, and high performance chromatography. The tested isolation gave the same result as the ammonia test for ultraviolet rays by changing the color of the inverted dish to red-carbon and expressing one isolation as a negative result of the test.
In the experiment, a bacteria (B. clausii) was more efficient, with a percentage of inhibition of 58.03 percent, while a bacteria (B. subtilis) had a percentage of inhibition of (55.28 percent), so it was chosen in our study to test its efficiency as a probiotic to remove toxins in rats. So 12 rats were divided into four groups, each with three rats. The first group was the control group, which was given only food and water, and the second group was given (0.5) ml of aflatoxin poison for each rat. For each rat, the third group received (0.5) ml of B clausii suspension and the fourth group received (0.5) ml of B. clausii suspension + (0.5) ml of aflatoxin toxin) for 21 days. The findings suggest that B.subtilis bacteria have effects. By increasing the weights of male rats inoculated with B.subtilis alone and the group (B.clausii + aflatoxin toxin), B.subtilis bacteria were able to remove a large percentage of the negative effects of aflatoxin.
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