Uncivil discourse is a growing concern in American rhetoric, and this trend has expanded beyond traditional media to online sources, such as audience comments. Using an experiment given to a sample representative of the U.S. population, we examine the effects online incivility on perceptions toward a particular issue—namely, an emerging technology, nanotechnology. We found that exposure to uncivil blog comments can polarize risk perceptions of nanotechnology along the lines of religiosity and issue support.

Intraductal carcinoma of the prostate and high grade prostatic intraepithelial neoplasia (PIN) have markedly different implications for patient care, but can be difficult to distinguish in needle biopsies. In radical prostatectomies, we demonstrated that PTEN and ERG immunostaining may be helpful to resolve this differential diagnosis. Here, we tested whether these markers are diagnostically useful in the needle biopsy setting. Separate or combined immunostains were applied to biopsies containing morphologically-identified intraductal carcinoma, PIN or borderline intraductal proliferations more concerning than PIN, but falling short of morphologic criteria for intraductal carcinoma. Intraductal carcinoma occurring with concurrent invasive tumor showed the highest rate of PTEN loss, with 76% (38/50) lacking PTEN and 58% (29/50) expressing ERG. Of biopsies containing isolated intraductal carcinoma, 61% (20/33) showed PTEN loss and 30% (10/33) expressed ERG. Of the borderline intraductal proliferations, 52% (11/21) showed PTEN loss and 27% (4/15) expressed ERG. Of the borderline cases with PTEN loss, 64% (7/11) had carcinoma in a subsequent needle biopsy specimen, compared to 50% (5/10) of PTEN-intact cases. In contrast, none of the PIN cases showed PTEN loss or ERG expression (0/19). On needle biopsy, PTEN loss is common in morphologically identified intraductal carcinoma yet is very rare in high grade PIN. Borderline intraductal proliferations, especially those with PTEN loss, have a high rate of carcinoma on resampling. If confirmed in larger prospective studies, these results suggest that PTEN and ERG immunostaining may provide a useful ancillary assay to distinguish intraductal carcinoma from high grade PIN in this setting.

Conflict in online discussions of science has the potential to polarize individuals’ perceptions of science, yet science communication scholarship has paid little attention to systematic study of how verbal attacks play out in online discussions of science. This study analyzes sarcasm and incivility in Twitter discussions of climate change during an extreme weather event ( n = 4,094). We found instances of incivility and sarcasm were low overall. Incivility was used in association with political topics, and both incivility and sarcasm were used alongside skeptical perspectives of climate change and by those who mention right-leaning politics in their profiles.

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