In this study, a cost and time saving strategy for the recovery of biomethane from rice straw using a novel phase-separation delignification process prior to a bacterial pretreatment (BP) has...
A halophilic bacterial consortium was enriched from Red Sea saline water and sediment samples collected from Abhor, Jeddah, Saudi Arabia. The consortium potentially degraded different low (above 90% for phenanthrene and fluorene) and high (69 ± 1.4 and 56 ± 1.8% at 50 and 100 mg/L of pyrene) molecular weight polycyclic aromatic hydrocarbons (PAHs) at different concentrations under saline condition (40 g/L NaCl concentration). The cell hydrophobicity (91° ± 1°) and biosurfactant production (30 mN/m) confirmed potential bacterial cell interaction with PAHs to facilitate biodegradation process. Co-metabolic study with phenanthrene as co-substrate during pyrene degradation recorded 90% degradation in 12 days. The consortium in continuous stirred tank reactor with petroleum refinery wastewater showed complete and 90% degradation of low and high molecular weight PAHs, respectively. The reactor study also revealed 94 ± 1.8% chemical oxygen demand removal by the halophilic consortium under saline condition (40 g/L NaCl concentration). The halophilic bacterial strains present in the consortium were identified as strain CEES1 (KX377976), strain CEES2 (KX377977), strain CEES3 (KX377978) and strain CEES4 (KX377979). Thus, the promising halophilic consortium was highly recommended to be employed in petroleum saline wastewater treatment process.
Single chamber air cathode microbial fuel cell (MFC) is a promising and sustainable technology to generate electricity. In the present study, the potential of air cathode MFC treating dye processing wastewater was investigated at various organic loads with interest focused on power densities, organic removal and coulombic efficiencies. The highest power density of about 515 mW/m 2 (6.03 W/m 3) with 56% of coulombic efficiency was procured at 1.0 (g COD/L) organic load. The high potency of TCOD (total chemical oxygen demand), SCOD (soluble chemical oxygen demand) and TSS (Total Suspended Solids) removal of about 85%, 73% and 68% respectively was achieved at the organic load of 1.0 (g COD/L). The bacterial strains in anode region at the initial stage of MFC operation were reported to be responsible for potential organic removal. The bacterial strains in air cathode MFC were identified as Paenibacillus sp. strain JRA1 (MH27077), Pseudomonas sp. strain JRA2 (MH27078), Ochrobactrum sp. strain JRA3 (MH27079), Sphingobacterium sp. strain JRA4 (MH27080), Stenotrophomonas sp. strain JRA5 (MH27081), Bacillus sp. strain JRA6 (MH27082) and Clostridium sp. strain JRA7 (MH27083) using phylogenetic analysis. After 60 days of air cathode MFC operation, the bacterial community in biofilm samples was dominated by Bacillus, Ochrobactrum and Pseudomonas (20-22%). The biofilm sample collected from the carbon brush consisted of Bacillus (33%), Ochrobactrum (30%), Pseudomonas (28%), Clostridium (6%) and Stenotrophomonas (3%). The present study revealed the treatment efficiency of dye processing wastewater along with power generation in single chambered air cathode MFC.
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