Microvascular anastomosis is a common part of many reconstructive and transplant surgical procedures. While venous anastomosis can be achieved using microvascular anastomotic coupling devices, surgical suturing is the main method for arterial anastomosis. Suture-based microanastomosis is time-consuming and challenging. Here, we fabricated dissolvable sugar-based stents as an assistive tool for facilitating surgical anastomosis. The non-brittle sugar-based stent holds the vessels together during the procedure and will be dissolved upon the restoration of the blood flow. The incorporation of sodium citrate minimized the chance of thrombosis. The dissolution rate and the mechanical properties of the sugar-based stent can be tailored between 4 to 8 minutes. To enable the fabrication of stents with desirable geometries and dimensions, three-dimensional (3D) printing was utilized to fabricate the stents. The effectiveness of the printed sugar-based stent was assessed ex vivo. The fabrication procedure is fast and can be performed in the operating room.
The ability to generate
chemical and mechanical gradients on chips
is important for either creating biomimetic designs or enabling high-throughput
assays. However, there is still a significant knowledge gap in the
generation of mechanical and chemical gradients in a single device.
In this study, we developed gradient-generating microfluidic circuits
with integrated microchambers to allow cell culture and to introduce
chemical and mechanical gradients to cultured cells. A chemical gradient
is generated across the microchambers, exposing cells to a uniform
concentration of drugs. The embedded microchamber also produces a
mechanical gradient in the form of varied shear stresses induced upon
cells among different chambers as well as within the same chamber.
Cells seeded within the chambers remain viable and show a normal morphology
throughout the culture time. To validate the effect of different drug
concentrations and shear stresses, doxorubicin is flowed into chambers
seeded with skin cancer cells at different flow rates (from 0 to 0.2
μL/min). The experimental results show that increasing doxorubicin
concentration (from 0 to 30 μg/mL) within chambers not only
prohibits cell growth but also induces cell death. In addition, the
increased shear stress (0.005 Pa) at high flow rates poses a synergistic
effect on cell viability by inducing cell damage and detachment. Moreover,
the ability of the device to seed cells in a 3D microenvironment was
also examined and confirmed. Collectively, the study demonstrates
the potential of microchamber-embedded microfluidic gradient generators
in 3D cell culture and high-throughput drug screening.
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