The
establishment of rapid targeted identification and analysis
of antibiotic resistance genes (ARGs) is very important. In this study,
an electrochemical sensor, which can detect ARGs was obtained by modifying
the sulfhydryl single-stranded DNA probe onto the thin-film gold electrode
through self-assembly. The sensor can perform a hybridization reaction
with a target sequence to obtain an electrochemical impedance spectroscopy
signal. The results showed that when the concentration of the probe
used to modify thin-film gold electrodes during preparation was 1
μM, the hybridization time was 1 h, and the hybridization temperature
was 35 °C, the self-assembled sensor showed good detection performance
for the ARGs encoding β-lactam hydrolase. The measurement ARG
concentration linear range is 6.3–900.0 ng/mL, and the R
2 is 0.9992. The sensor shows good specific
recognition ability for single-base, double-base, and three-base mismatch
DNA. In addition, after 30 days of storage at 4 °C, the accurate
identification and analysis of ARGs can still be maintained.
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