An algorithm for post-processing of the grayscale 3D computed tomography (CT) images of porous structures with the automatic selection of filtering parameters is proposed. The determination of parameters is carried out on a representative part of the image under analysis. A criterion for the search for optimal filtering parameters based on the count of "levitating stone" voxels is described. The stages of CT image filtering and its binarization are performed sequentially. Bilateral and anisotropic diffuse filtering is implemented; the Otsu method for unbalanced classes is chosen for binarization. Verification of the proposed algorithm was carried out on model data. To create model porous structures, we used our image generator, which implements the function of anisotropic porous structures generation. Results of the post-processing of real CT images containing noise and reconstruction artifacts by the proposed method are discussed.
The human olfactory bulb (OB) has a laminar structure. The segregation of cell populations in the OB image poses a significant challenge because of indistinct boundaries of the layers. Standard 3D visualization tools usually have a low resolution and cannot provide the high accuracy required for morphometric analysis. X-ray phase contrast tomography (XPCT) offers sufficient resolution and contrast to identify single cells in large volumes of the brain. The numerous microanatomical structures detectable in XPCT image of the OB, however, greatly complicate the manual delineation of OB neuronal cell layers. To address the challenging problem of fully automated segmentation of XPCT images of human OB morphological layers, we propose a new pipeline for tomographic data processing. Convolutional neural networks (CNN) were used to segment XPCT image of native unstained human OB. Virtual segmentation of the whole OB and an accurate delineation of each layer in a healthy non-demented OB is mandatory as the first step for assessing OB morphological changes in smell impairment research. In this framework, we proposed an effective tool that could help to shed light on OB layer-specific degeneration in patients with olfactory disorder.
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