BackgroundIndonesian mistletoe grows on various trees. Mango Mistletoes (Dendrophthoe pentandra) is one type of mistletoe that grown on mango tree (.benalu mangga in bahasa Indonesia). Our study used mistletoe as a parasitic plant that has been used for traditional medicine. It has been known that Dendrophtoe pentandra extract (DPE) anti-inflammatory and anticancer. Furthermore, it is necessary to follow-up study in vivo to evaluate the response to treatment of new cancer therapeutic agents. This research aimed to determine the levels of IL-22, myeloperoxide (MPO), proliferation and wild-type p53 expression after the administration of DPE to murine models of CAC.MethodsMouse colitis associated colon cancer (CAC) was induced firstly by azoxymethane (AOM) and followed by administration of drinking water containing 5 % dextran sodium sulfate (DSS) in a cycle protocol, each cycle consisted of seven days of 5 % DSS in the drinking water and followed by seven days of regular water. This study consists of five treatment groups: I was treated water only (control), II was administrated by (DSS only, without DPE), (III-V) were administrated by DPE (125 mg/kg BW, 250 mg/kg BW and 500 mg/kg BW) respectively. The administrated of DPE were started from the 8th weeks, were continued until 21 weeks. At the end of 21 weeks of the experiment, mice were sacrificed, colon tissue was removed, and then subjected to ELISA, flow cytometry, real-time PCR and histology examination.ResultsAdministration of DPE 250 mg/kgBW significantly reduce the levels of IL-22 and MPO compared with DSS only group (p < 0.001; p < 0.001). Colonic epithelial cells proliferation of group IV (DPE 250 mg/kgBW) were significantly lower than III and V groups. There was no significant change in the S phase in mice were treated DPE 125 mg/kg BW and 500 mg/kg BW, while administration of DPE 250 mg/kg BW was able to increase the percentage of cells in S phase. The expression of mRNA p53 was up regulated in mice received DPE 125 mg/kg BW.ConclusionThese findings indicate that the DPE could inhibit colonic epithelial cells proliferation through p53 pathway independently. This study also showed that DPE could be potential sources of new therapy.
Brotowali (Tinospora crispa L) is a plant which has potential to be a chemopreventive agent. This study aims to determine the profile of anticancer activity of brotowali stem extracts (Tinospora crispa L.), from several regions in East Java. The extraction was carried out by maceration method using 80% ethanol solvent. Then, anticancer activity test was carried out on MCF-7 breast cancer cell model using the Microtetrazolium (MTT) Assay method. The results of the anticancer activity test showed that 15 brotowali stem extracts taken from 5 locations in East Java had significant differences in anticancer activity (P <0.05). Brotowali extracts from Kanigoro, Blitar city, had the highest anticancer activity with an IC50 value of 30.64 µg / mL.
Macrosolen cochinensis is a parasite grows in Indonesia and is known to possess the anticancer activity. This study aims to explain the anticancer activity of the extracts and fractions of jackfruit mistletoe leaves and describe the cell cycle inhibition and induction of apoptosis on T47D breast cancer cell line. The jackfruit mistletoe is extracted using the maceration method. The extract is then separated by liquid-liquid partition method with n -hexan, chloroform, ethyl acetate, and water solvent. MTT method is used to determine the anticancer activity of crude extract and fractions. Cell cycle inhibition test is conducted using flow cytometry with PI marker. The induction of apoptosis is determined by flow cytometry method using PIAnnexin V and PI double staining acridine orange-ethidium bromide. The results indicated that the IC 50 value of the ethanol extract, chloroform fractions and ethyl acetate fractions of jackfruit mistletoe leaves showed a higher anticancer activity with IC 50 which respectively are 362.8, 356.8, 314.8μg/ml. The treatment of n -hexan and water fraction show no signs of anticancer activity because it has a great value of IC 50 which are respectively 926.0 and 2243 μg/ml. Cell death caused by treatment of ethyl acetate fraction of jackfruit mistletoe leaf (Macrosolen cochinensis) is due to the induction of cell apoptosis and cell cycle inhibition in G0-G1, S and G2-M phase.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.