Mitochondria and chloroplasts are organelles with high iron demand that are particularly susceptible to iron-induced oxidative stress. Despite the necessity of strict iron regulation in these organelles, much remains unknown about mitochondrial and chloroplast iron transport in plants. Here, we propose that Arabidopsis ferroportin 3 (FPN3) is an iron exporter that is dual-targeted to mitochondria and chloroplasts. FPN3 is expressed in shoots, regardless of iron conditions, but its transcripts accumulate under iron deficiency in roots. fpn3 mutants cannot grow as well as the wild type under iron-deficient conditions and their shoot iron levels are lower compared with the wild type. Analyses of iron homeostasis gene expression in fpn3 mutants and inductively coupled plasma mass spectrometry (ICP-MS) measurements show that iron levels in the mitochondria and chloroplasts are increased relative to the wild type, consistent with the proposed role of FPN3 as a mitochondrial/plastid iron exporter. In iron-deficient fpn3 mutants, abnormal mitochondrial ultrastructure was observed, whereas chloroplast ultrastructure was not affected, implying that FPN3 plays a critical role in the mitochondria. Overall, our study suggests that FPN3 is essential for optimal iron homeostasis.

Mitochondria and chloroplasts are organelles with high iron demand that are highly susceptible to iron-induced oxidative stress. Despite the necessity of strict iron regulation in these organelles, much remains unknown about mitochondrial and chloroplast iron transport in plants. Here, we show that Arabidopsis Ferroportin 3 (FPN3) is an iron exporter dual-targeted to mitochondria and chloroplasts. FPN3 is expressed in shoots regardless of iron conditions, but is iron-regulated in the roots. fpn3 mutants cannot grow as well as wild type under iron-deficient conditions and shoot iron levels are reduced in fpn3 mutants compared to wild type. Analyses of iron homeostasis gene expression in fpn3 mutants and ICP-MS measurements suggest that iron levels in the mitochondria and chloroplasts are increased relative to wild type, consistent with the proposed role of FPN3 as a mitochondrial/plastid iron exporter. In iron deficient fpn3 mutants, abnormal mitochondrial ultrastructure was observed, whereas chloroplast ultrastructure was not affected, implying that FPN3 plays a critical role in iron metabolism in mitochondria. Overall, we propose that FPN3 is crucial for iron homeostasis.One sentence summaryFPN3 is an iron exporter dual-targeted to the mitochondria and plastids and is required for optimal growth under iron deficiency.

Various problems often arise in high-rise buildings during the winter months due to the stack effect. In this study, the high-rise building of interest, located in South Korea, was experiencing constant loud noises in the winter due to the stack effect. Thus, we created a noise level reduction plan by creating a method for pressurizing the high-rise zones of the building according to outdoor conditions. To discover the appropriate pressurization operating modes, we applied a two-year commissioning process to the 50-story building of interest. The 1st- and 47th-floor elevator halls were identified to have the highest noise levels of all other floors. Prior to applying the reduction plan, the maximum noise level on the first floor with the HVAC system turned off was 85 dB(A) and with the HVAC system turned on it was 70 dB(A). Both values exceeded the criteria of 57 dB(A) for a lobby space of a commercial building. In the case of the 47th floor, the maximum noise level with the HVAC system turned off was 58.7 dB(A) and with the HVAC system turned off was 56.0 dB(A), despite the latter having increased airtightness performance and applying preliminary pressurization (i.e., HVAC operation mode 2). These values exceeded the criteria of 48 dB(A) for an elevator hall in a commercial building. Following this initial data, we determined to pressurize the high/mid-rise zones of the building according to the outdoor air temperature and wind velocity conditions, which we categorized into four types (i.e., HVAC operation mode 4). To this effect, the first-floor elevator hall’s maximum noise level was 56.6 dB(A), meeting the criteria, and the 47th-floor elevator hall’s maximum noise level was 49.5 dB(A), still exceeding the criteria but by an insignificant amount. Although the HVAC pressurization operation we utilized resulted in favorable results for the target building A, it may not be as effective in other new high-rise buildings, creating changes to the indoor air environment or to the energy costs in maintaining a building. However, for the purposes of resolving the stack effect, we believe that the commissioning process we took to optimize the HVAC operation that is presented here can be applied to other new and existing high-rise commercial buildings.