We identified the near-full-genome sequence (29,908 nt, >99%) of Middle East respiratory syndrome coronavirus (MERS-CoV) from a nasal swab specimen from a dromedary camel in Egypt. We found that viruses genetically very similar to human MERS-CoV are infecting dromedaries beyond the Arabian Peninsula, where human MERS-CoV infections have not yet been detected.

Results of this study indicate that the number of humans infected with avian influenza viruses is much larger than the number of reported confirmed cases. In an area where these viruses are enzootic in the poultry, human exposure to and infection with avian influenza becomes more common.

SARS-CoV-2 virus is transmitted in closed settings to people in contact with COVID-19 patients such as healthcare workers and household contacts. However, household person-to-person transmission studies are limited. Households participating in an ongoing cohort study of influenza incidence and prevalence in rural Egypt were followed. Baseline enrollment was done from August 2015 to March 2017. The study protocol was amended in April 2020 to allow COVID-19 incidence and seroprevalence studies. A total of 290 households including 1598 participants were enrolled and followed from April to October 2020 in four study sites. When a participant showed respiratory illness symptoms, a serum sample and a nasal and an oropharyngeal swab were obtained. Swabs were tested by RT-PCR for SARS-CoV-2 infection. If positive, the subject was followed and swabs collected on days three, six, nine, and 14 after the first swab day and a serum sample obtained on day 14. All subjects residing with the index case were swabbed following the same sampling schedule. Sera were collected from cohort participants in October 2020 to assess seroprevalence. Swabs were tested by RT-PCR. Sera were tested by Microneutralization Assay to measure the neutralizing antibody titer. Incidence of COVID-19, household secondary attack rate, and seroprevalence in the cohort were determined. The incidence of COVID-19 was 6.9% and the household secondary attack rate was 89.8%. Transmission within households occurred within two-days of confirming the index case. Infections were asymptomatic or mild with symptoms resolving within 10 days. The majority developed a neutralizing antibody titer by day 14 post onset. The overall seroprevalence among cohort participants was 34.8%. These results suggest that within-household transmission is high in Egypt. Asymptomatic or mild illness is common. Most infections seroconvert and have a durable neutralizing antibody titer.

Given the importance of influenza infections in the Eastern Mediterranean Region (EMR), we conducted a comprehensive literature review to analyze the status of influenza research in the region from 2012. Influenza research has gained more momentum recently with the emergence of H5N1 and new virus strains. Research covering epidemiological, veterinary, and basic science aspects is growing. More sequences were being generated per year, not only for diagnostic purposes but also for research. We included gray literature publications in our search and found several graduate student dissertations from Egypt, which were published on an online portal. However, the search revealed some weaknesses, mostly in the areas of study design and the lack of surveillance studies. Another weakness was the fact that the publications originated from very few countries, mainly Egypt and Iran. Although improving, influenza research in the EMR remains weak. We recommend encouraging countries in the EMR to conduct more influenza research using stronger methodologies.

BACKGROUND: Lockdown and school closure related to the coronavirus disease 2019 (COVID-19) have unfavorable effects on children and adolescents. AIM: This study was conducted to survey the changes in dietary pattern and related health factors in Egyptian children and adolescents during the COVID-19 outbreak closure. MATERIALS AND METHODS: Data were collected through a dietary pattern, eating behavior, and physical activity electronic questionnaire conducted through social media sites, targeting parents of children and adolescents after two whole months of lockdown and school closure in Egypt. RESULTS: This study included 765 participants, 31.8% noted increased appetite, 45.6% reported increased sweets and unhealthy food consumption, and 37.6% showed increased frequent snacking between meals. Alongside 53.1% showed increase in late snacks during night after COVID-19 closure. The majority of our participants 82.0% noted associative change in eating behavior with boring and 94.6% revealed increased usage of electronics and screen time. This study showed significant positive correlation between increased appetite and mobile screen time, laptop screen time, and video gaming. Increased sweets and unhealthy food consumption was positively correlated with TV watching and mobile screen time. A significant positive correlation was revealed between uncaring about eating fruits and vegetables and increase screen time for each of mobile, and laptop and remote learning. Furthermore, there was positive correlation between decreased protein serving intake and each of mobile screen time and remote learning. TV watching and laptop screen times showed positively significantly association with frequent snacking between meals. Mobile screen time, TV screen time, and video gaming were positively significantly correlated with late night snacking. CONCLUSIONS: The present study concluded prolonged lockdown leads to changes in eating patterns, related to contributing factors of physical inactivity and prolonged screen time.