Background:The aim of the study was to evaluate the potential of manganese-zinc ferrite nanoparticles (MZF NPs) as a novel negative magnetic resonance imaging (MRI) contrast agents for 4T1 (mouse mammary carcinoma) and L929 (murine fibroblast) cell lines.Methods:MZF NPs and its suitable coating, polyethylene glycol (PEG) via covalent bonding, were investigated under in vitro condition. The cytotoxicity of MZF NPs was tested by 3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide assay after 12 and 24 h of incubation. To evaluate the potential of MZF NPs as T2 MRI nanocontrast agent, images were obtained from phantom containing different Fe concentrations and T2 relaxivity (r2) was measured. The viability of both 4T1 breast cancer and L929 murine fibroblast cell lines incubated with different Fe concentrations.Results:In vitro T2-weighted MRI showed that signal intensity of 4T1 cells was lower than that of L929 as control cells. T2-weighted MRI showed that signal intensity of MZF NPs enhanced with increasing concentration of NPs. The values of 1/T2 relaxivity (r2) for coated MZF NPs with PEG found to be 85.5 mM−1 s−1 which is higher than that of commercially clinical used (Sinerem) MRI contrast agent.Conclusion:The results showed that MZF NPs have potential to detect breast cancer cells (4T1) and also have high contrast resolution between normal (L929) and cancerous cells (4T1) which is a suitable nanoprobe for T2-weighted MR imaging contrast agents.
Objective:
Poly dopamine coated iron oxide nanoparticles (Fe3O4@PDA NPs) were
synthesized, characterized, and their MR imaging contrast agents and photothermal potency
were evaluated on melanoma (B16-F10 and A-375) cells and normal skin cell. To this end,
MTT assay, Fe concentration, and MR imaging of both coated and uncoated NPs were
assessed in C57BL/6 mice.
Methods:
Fe3O4 nanoparticles were synthesized using co-precipitation, and coated with poly
dopamine. The cytotoxicity of Fe3O4 and Fe3O4@PDA NPs on melanoma cells with different
concentrations were obtained using MTT assay. MR images and Fe concentration, of
nanoprobe and nanoparticles were evaluated under in vivo conditions.
Results:
Findings indicated that uncoated Fe3O4 showed the highest toxicity on animal (B16-
F10) cells at 450µg/ml after 72h, while the highest toxicity in human (A-375) cells were
observed at 350µg/ml. These nanoparticles did not reveal any cytotoxicity to normal skin
cells, despite having some toxicity features in A-375 cells. MR image signals in tumor were
low compared with other tissues. The iron concentration in tumor was higher than that of
other organs.
Conclusion:
It is concluded that the cytotoxicity of Fe3O4@PDA were found to be
significantly lower than uncoated nanoparticles (p <0.001) which allow some positive effects
on reducing toxicity. The prepared nanoprobe may be used as contrast agent in MR imaging.
Polyoxometalates (POMs) are mineral nanoclusters with many advantages in various diagnostic fields, in particular cancer detection. This study aimed to synthesize and evaluate the performance of gadolinium–manganese–molybdenum polyoxometalate (Gd-Mn-Mo; POM) nanoparticles coated with chitosan–imidazolium (POM@CSIm NPs) for detecting 4T1 breast cancer cells by magnetic resonance imaging in vitro and in vivo. The POM@Cs-Im NPs were fabricated and characterized by FTIR, ICP-OES, CHNS, UV–visible, XRD, VSM, DLS, Zeta potential, and SEM. Cytotoxicity, cellular uptake, and MR imaging in vivo and in vitro of L929 and 4T1 cells were also assessed. The efficacy of nanoclusters was demonstrated using MR images of BALB/C mice bearing a 4T1 tumor in vivo. The evaluation of the in vitro cytotoxicity of the designed NPs showed their high biocompatibility. In fluorescence imaging and flow cytometry, NPs had a higher uptake rate by 4T1 than L929 (p < 0.05). Furthermore, NPs significantly increased the signal strength of MR images, and its relaxivity (r1) was calculated as 4.71 mM−1 s−1. MR imaging also confirmed the attachment of nanoclusters to cancer cells and their selective accumulation in the tumor region. Overall, the results showed that fabricated POM@CSIm NPs have considerable potential as an MR imaging nano-agent for early 4T1 cancer detection.
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