In this study, we characterized Hypericum vesiculosum Griseb. (sect. Drosocarpium), Hypericaceae, a Balkan endemic and Greek subendemic species, which was collected from the Peloponnese (Greece). The anatomical study showed that its leaves are dorsiventral and hypostomatic, and the major part of its circular stem is filled with xylem. High-performance liquid chromatography coupled with diode-array detection and mass spectrometry analysis of the methanolic extract of the aerial parts revealed 25 compounds, and the concentration of the 18 major ingredients was determined with a validated method of liquid chromatography coupled with diode-array detection. Flavonoids were the most abundant group of compounds (> 100 mg/g of dry extract) with I3,II8-biapigenin, apigenin hexoside, isoquercitrin, quercetin, and hyperoside as the major dereplicated constituents. Chlorogenic acid was abundant (9.34 ± 0.22 mg/g) and the content of naphthodianthrones was comparable to that in H. perforatum. Only one phloroglucinol could be quantified at a very low concentration and that was not hyperforin. This is the first chemical analysis of its methanolic extract and anatomical characterization of its vegetative tissues.
The promyelocytic leukemia protein (PML) is the core organizer of the cognate nuclear bodies (PML-NBs). Through physical interaction or modification of diverse protein clients, PML-NBs regulate a multitude of - often antithetical- biological processes such as antiviral and stress response, inhibition of cell proliferation and autophagy, and promotion of apoptosis or senescence. Although PML was originally recognized as a tumor-suppressive factor, more recent studies revealed a “double faced” agent role for PML. Indeed PML displayed tumor cell pro-survival and pro-migratory functions via inhibition of migration suppressing molecules or promotion of transforming growth factor beta (TGF-β) mediated Epithelial-Mesenchymal Transition (EMT) that may promote cancer cell dissemination. In this line, PML was found to correlate with poor patient prognosis in distinct tumor contexts. Furthermore in the last decade, a number of publications have implicated PML in the physiology of normal or cancer stem cells (CSCs). Promyelocytic leukemia protein is activating fatty acid oxidation (FAO), a metabolic mechanism required for the asymmetric divisions and maintenance of hematopoietic stem cells (HSCs). In embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), PML is required for maintenance of the naïve and acquisition of the induced pluripotency state respectively. Correspondingly, PML ablation causes significant morphological, gene expression and lineage choice changes. In this review, we focus on the mechanisms orchestrated by PML and PML-NBs in cancer and healthy stem cells from cell physiology to the regulation of chromatin dynamics.
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