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1. Whole cell patch clamp recordings of neurons in slices of the suprachiasmatic nucleus (SCN) were made in order to assess their electrophysiological and morphological heterogeneity. This assessment was accomplished by (i) quantification of intrinsic membrane properties recorded in current clamp mode, (ii) studying frequency distributions of these properties, (iii) grouping of cells based on visual inspection of data records, and (iv) use of cluster analysis methods. 2. Marked heterogeneity was found in the resting membrane potential, input resistance, time constant, rate of frequency adaptation, size of rebound depolarization (low-threshold Ca2+ potential) and regularity of firing. The frequency distribution of these membrane properties deviated significantly from a normal distribution. Other parameters, including spike amplitude and width, amplitude and rising slope of the spike after-hyperpolarization (AHP) and amplitude of the spike train AHP, showed considerable variability as well but generally obeyed a normal distribution. 3. Visual inspection of the data led to partitioning of cells into three clusters, viz. cluster I characterized by monophasic spike AHPs and irregular firing in the frequency range from 1.5 to 5.0 Hz; cluster II with biphasic spike AHPs and regular firing in the same range; and cluster III with large rebound depolarizations and biphasic spike AHPs. In a post hoc analysis, these clusters also appeared to differ in other membrane properties. This grouping was confirmed by hierarchical tree clustering and multidimensional scaling. 4. The light microscopic properties of recorded neurons were studied by biocytin labelling. Neurons had monopolar, bipolar or multipolar branching patterns and were often varicose. Axons sometimes originated from distal dendritic segments and usually branched into multiple collaterals. Many cells with extra-SCN projections also possessed intranuclear axon collaterals. We found no morphological differences between clusters except that cluster III neurons possessed more axon collaterals than cluster I or II cells. 5. These results suggest that SCN neurons are heterogeneous in some basic as well as active membrane properties and can be partitioned into at least three clusters. Cluster I and II cells fire spontaneously in a regular and irregular mode, respectively, and sustain prolonged spike trains. In contrast, cluster III cells have low firing rates but may adopt a burst-like firing mode when receiving appropriate input. While all clusters transmit output to target cells within and outside SCN, cluster III cells in particular are suggested to affect excitability of large numbers of SCN neurons by their extensive local network of axon collaterals.

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Circadian oscillator mechanisms in the suprachiasmatic nucleus (SCN) can be reset by photic input, which is mediated by glutamatergic afferents originating in the retina. A key question is why light can only induce phase shifts of the biological clock during a restricted period of the circadian cycle, namely the subjective night. One of several possible mechanisms holds that glutamatergic transmission at retinosuprachiasmatic synapses would be altered, in particular the contribution of glutamate receptor subtypes to the postsynaptic response. By studying the contributions of NMDA and non‐NMDA glutamate receptors to the retinal input to SCN in whole‐cell patch‐clamp recordings in acutely prepared slices, we tested the hypothesis that NMDA receptor current evoked by optic nerve activity is potentiated during the subjective night. During the day the NMDA component of the EPSC evoked by optic nerve stimulation was found less frequently and was significantly smaller in magnitude than during the night. In contrast, the non‐NMDA component did not show a significant day‐night difference. When the magnitude of the NMDA component was normalized to that of the non‐NMDA component, the day‐night difference was maintained, suggesting a selective potentiation of NMDA receptor conductance. In addition to contributing to electrically evoked EPSCs, the NMDA receptor was found to sustain a small, tonically active inward current during the night phase. No significant tonic contribution by NMDA receptors was detected during the day. These results suggest, first, a dual mode of NMDA receptor function in the SCN and, second, a clock‐controlled type of receptor plasticity, which may gate the transfer of photic input to phase‐shifting mechanisms operating at the level of molecular autoregulatory feedback loops.