e papilloma viruses are constituted of double-stranded DNA and are a more common lesion in ruminant's skin in Iraq. e p53 tumor suppressor protein reveals an essential role in cell cycle control. is study intends to investigate the clinical, histopathological, and immunohistochemical features of cutaneous papilloma in ruminants in Iraq. Samples had been collected from a total of 10 animals (three cattle, three goats, and four sheep) with multiple papillomatosis lesions. e samples were processed for histopathological and immunohistochemical techniques. Clinically, the lesions appeared as multiple various sizes (0.5-11 cm), cauliflower exophytic masses on different parts of the animal's body. e histopathological features of the epidermis granular layer revealed perinuclear vacuolation (koilocytosis) accompanied by various degrees of hypergranulosis, hyperkeratosis, acanthosis, orthokeratosis, and parakeratosis. Strong positive reaction for papillomavirus antigen was seen in both epidermal basal and granular layers in the immunohistochemical investigation (IHC). Moreover, all papilloma lesions revealed an intense positive p53 reaction in cytoplasmic and perinuclear of the basal and parabasal layers. In conclusion, this study described the papillomavirus lesions in bovine, ovine, and caprine animals, which were found in different parts areas of the affected animals. All lesions show similar histopathological features with minor variations. PV antigen and p53 protein expression showed positive results in immunohistochemistry that can be used as diagnostic markers for ruminant's papilloma.
This is a case report describing application of Amniotic Membrane (AM) in the treatment of penetrated dog's bite wounds in two sheep. The AM was collected from a goat during caesarean section and inserted into the bite injuries after preparation. Both cases were followed up until healing. Complete regeneration with normal histological features was evident after six weeks.An interesting effect of AM on regeneration of the bite wounds was apparent and to our knowledge, this is the first report concerning treatment of dog's bite wounds by AM.
Pyloromyotomy is a procedure to increase the diameter of pyloric region, routinely performed during pyloric stenosis to prevent delayed emptying of stomach content. The aim of this study was to evaluate the pyloromyotomy by conventional and laparoscopic techniques. Ten adult healthy dogs were used. Five animals underwent the pyloromyotomy by conventional and the same number laparoscopic technique. The clinical radiological and laparoscopic results of two techniques revealed that both were successive for achieved this purpose, but the laparoscopic pyloromoytomy characterized by small abdominal incision minimal adhesion and rapid healing.
A five female calves about (2-5) months old were presented with congenital atresia ani and recto vaginal fistula; these were clinically examined and recognized as a congenital anomalies. Treatment was effective. Under caudal epidural anesthesia was given with 2% Lidocaine HCL, the anal orifice was reconstructed and the fistulous tract was closed successfully. The rectovaginal fistula was repaired by placing continuous sutures in roof of vagina. Penicillin Streptomycin was injected postoperatively at a dose 10000 IU and 10mg/kg B.W intramuscularly respectively, and the anal stitches were removed after 14 days.
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