The potential of phenolic compounds of medicinal plants including Astragalus danicus L is determined by but not limited to their antioxidant activity. Their anti-inflammatory, antitumor, and other useful properties are known, which allows using these phytochemicals within preventive activities to reduce the risk of many serious diseases. Chromatographic analysis of the Astragalus danicus L. biomaterial from the plant samples collected in three regions of the Kemerovo region (Western Siberia, Russia) established the presence of compounds of flavonols (isorhamnetin glucoside, kaempferol glucoside), flavones (apigenin 7-glucoside), phenylpropanoids (chlorogenic acid) in the aerial part of plants. The total content of phenolic compounds in plant samples ranged from 100.75 ± 3.87 mg/g (Yashkinsky district) to 190.95 ± 7.34 mg/g (Belovsky district). The content of chlorogenic acid in the studied samples was from 0.14 ± 0.01 mg/g to 1.16 ± 0.04 mg/g. Isorhamnetin glucoside was found only in samples of plants from two districts - Prokopievsky (41.39 ± 1.58 mg/g) and Belovsky (95.0 ± 3.66 mg/g). The content of glucosides of kaempferol ranged from 0.38 ± 0.01 mg/g to 0.55 ± 0.02 mg/g. Its content is almost twice as high as the content in the well-known analogues of Astragalus. Apigenin-7-glucoside was isolated in Astragalus samples for the first time, in a small amount (3.34 ± 0.13 mg/g) in a sample of plants of one growing zone. Studies have confirmed that the content of flavonoids in plants significantly depends not only on the genetic characteristics of plants, but also on the hydrothermal regime, the climatic conditions of different botanical and geographical areas of the habitat. This work shows that Astragalus danicus L. growing in Kemerovo region is a promising raw material for pharmacological preparations.
Immunodeficiency causes a lot of modern diseases. Immunodeficiency, in its turn, is caused by such fac- tors as polluted environment, chronic stress, sedentary lifestyle, unbalanced diet, etc. All these factors weaken respi- ratory organs and gastrointestinal tract, disturb hormonal regulation, and destabilize immune defence. Food industry responds to these challenges by developing functional foods and dietary supplements from medicinal plants. Dietary supplements made from natural plant extracts have more advantages than their numerous synthetic analogues. They produce a mild therapeutic effect and no pronounced side effects. Purple Echinacea (Echinacea purpurea L.) posses- ses immunomodulatory, anti-inflammatory, antiviral, and tonic properties. However, climatic and soil conditions are known to affect the qualitative and quantitative profile of biologically active substances. The present paper describes the micronutrient profile of various parts of Echinacea purpurea grown in the Kemerovo region. The study employed a complex of physical and chemical methods. The research featured leaves, roots, and flowers, as well as components extracted from the plant with the help of a 70% ethanol solution. The latter was chosen for its universal properties in micronutrient extraction. The methods included high performance liquid chromatography (HPLC), thin layer chro- matography (TLC), and IR spectroscopy. A set of triple experiments showed that the extracts contained substances with anti-inflammatory, antioxidant, and immunomodulating properties. Thus, Echinacea extract can be recommen- ded for functional foods and dietary supplements.
Introduction. Modern scientific research into the biochemical composition and medicinal value of plants makes it possible to use them as functional ingredients in food technology. The research objective was to test rose root (Rhodiola rosea L.) and scullcap (Scutellaria galericulata L.) for biologically active substances and their potential use in functional dairy products. Study objects and methods. The research featured biologically active substances (BAS) obtained from rose root and scullcap that grow in mountain areas or on rock outcrops along Siberian rivers. The BAS content was determined using high performance liquid chromatography (HPLC). The biologically active substances were screened and identified using HPLC, thin-layer chromatography (TLC), and infra-red identification (IR). The new functional products were based on whey and cottage cheese made from processed whole milk. Results and discussion. The analysis of Rhodiola rosea rhizomes and roots showed the following BAS content (mg/g): rosavin – 16.9, salidroside – 14.3, rosin – 5.04, rosarin – 2.01, and methyl gallate – 6.8. The roots of Scutellaria galericulata had the following BAS content (mg/g): scutellarein – 22.27, baicalin – 34.37, baicalein – 16.30, apigenin – 18.80, chrysin – 6.50, luteolin – 5.40, and vogonin – 3.60. Whey served as a basis for a new functional whey drink fortified with BAS isolated from Rhodiola rosea 100 mL of the drink included 50 mL of whey, 20 mL of apple juice, 0.1 mL of rose root concentrate, 3 g of sugar, 0.5 g of apple pectin, 04 g of citric acid, and 30 mL of ionized water. The content of phytochemical elements ranged from 0.11 ± 0.001 to 0.49 ± 0.08 mg/100 g. Cottage cheese served as a basis for another dairy product fortified with BAS obtained from Scutellaria galericulata. The formulation included 81 g of cottage cheese, 10 mL of cherry jam, 9 g of sugar, and 0.025 mL of scullcap concentrate. The content of biologically active substances in the finished product varied from 0.09 ± 0.02 for luteolin to 0.48 ± 0.11 for baicalin. The whey drink fortified with the BAS extracted from Rhodiola rosea and the cottage cheese product fortified with the BAS isolated from Scutellaria galericulata satisfied 40–45% and 55–60% of the reference daily intake for phenolic compounds, respectively. The obtained data made it possible to recommend the new functional foods for commercial production. Conclusion. A set of experiments was performed to isolate biologically active substances from Rhodiola rosea and Scutellaria galericulata. The research developed and tested formulations of two new functional products based on whey and cottage cheese.
Biologically active substances extricated by extraction of water and 40% ethyl alcohol from rootstalks of Rhodiola rosea L.: gallic acid, forms of rosovin and salidroside have been studied. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used during the research. Determination of antimicrobial activity was performed by agar diffusion method (OFC.1.2.4.0010.15) [8]. The method is based on the evaluation of inhibition of the growth zone of testing cultures of microorganisms by certain concentrations of biologically active substances (BAS). String formed bacteria (8 strains), cocci bacteria (3) and fungal (4) microorganisms, bifidobacterium (10 strains) were used as testing cultures. Various responses of microorganisms to exposure by biologically active substances have been determined. Gallic acid and salidroside in the aqueous phase have the maximum inhibitory property to rod bacteria and coccoid microorganisms. Post-antibiotic effect is shown in rozovin. Salidroside in the butanol phase is more neutral to most microorganisms, with the exclusion of: Acinetobacter baumannii, Pseudomonas aeruginosa, Bacillus cereus and Staphylococcus aureus. The lack of a clearly expressed reaction of BAS to bifidobacterium, most probably, gives the possibility of their use in the development of functional products of a new generation. However, first of all, it is required to organize a special experiment on the structure of the introduction of the studied components into the lactoserum. Next, determine the content of BAS in the derived product, the reaction of bifidobacterium admitted for use in biotechnology, and a standard set of microbiological strains. Gallic acid is suggested to be introduced in applied studies to explore as a target immunomodulator of resistance to the listed microflora and to develop an effective form of a new product.
The technology of creating varieties of barley resistant to the smut diseases has been developed. Their cultivation will help to reduce crop losses, increase the profitability of seed production, improve the quality of seeds, and reduce the environmental risk from the use of chemical plant protection products. This technology was first implemented in the Kemerovo Region in 1988-1999. The wide distribution of species Ustilago nuda (Jens.) Kell. et Sw. (65 %) and U. nigra Tapke (35 %) was revealed. The covered smut ( Ustilago hordei (Pers.) Kell. Et Sw.) was registered in individual collection varieties. The first and tenth races of a loose smut and the sixth race of a black loose smut were identified. The sources of individual and group immunity to the smut fungi from the world gene pool of barley are distinguished: Moskovsky 3/125, Suzdalets, Ramos (Moscow Region); Kazer (Rostov Region); line 3 KM-1192 (Belarus); Kumir Odessky (Ukraine); Guardian (Canada). The donor properties of resistance to a loose smut in the Bagan variety, line 53 HVS 91/76, line 1899 were studied. They are recommended for use in breeding programs. With their participation a new hybrid fund was created. Variability and inheritance of quantitative traits of productivity of hybrids was studied. To select the recombinants immune to the loose smut in combination with high productivity, the most promising combinations of hybrids have been identified: Bagan × Viner; Bagan × KM-16; l. 53 HVS 91/76 × KM-7; KM-7 × l. 1899; l. 1899 × KM-7. Among them, prospective breeding lines have been selected. The results of the implementation of technology elements in the selection of barley for resistance to smut fungi is the creation of immune highly productive varieties of a new generation: Simon and Luka (Kemerovo Region). They are included in the State register of breeding achievements since 2004.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.