The development of biomedical science requires the creation of biological material collections that allow for the search and discovery of biomarkers for pathological conditions, the identification of new therapeutic targets, and the validation of these findings in samples from patients and healthy people. Over the past decades, the importance and need for biobanks have increased considerably. Large national and international biorepositories have replaced small collections of biological samples. The aim of this work is to provide a basic understanding of biobanks and an overview of how biobanks have become essential structures in modern biomedical research.

Bile acids are important physiological agents required for the absorption, distribution, metabolism, and excretion of nutrients. In addition, bile acids act as sensors of intestinal contents, which are determined by the change in the spectrum of bile acids during microbial transformation, as well as by gradual intestinal absorption. Entering the liver through the portal vein, bile acids regulate the activity of nuclear receptors, modify metabolic processes and the rate of formation of new bile acids from cholesterol, and also, in all likelihood, can significantly affect the detoxification of xenobiotics. Bile acids not absorbed by the liver can interact with a variety of cellular recipes in extrahepatic tissues. This provides review information on the synthesis of bile acids in various parts of the digestive tract, its regulation, and the physiological role of bile acids. Moreover, the present study describes the involvement of bile acids in micelle formation, the mechanism of intestinal absorption, and the influence of the intestinal microbiota on this process.

An athlete’s diet is influenced by external and internal factors that can reduce or exacerbate exercise-induced food intolerance/allergy symptoms. This review highlights many factors that influence food choices. However, it is important to remember that these food choices are dynamic, and their effectiveness varies with the time, location, and environmental factors in which the athlete chooses the food. Therefore, before training and competition, athletes should follow the recommendations of physicians and nutritionists. It is important to study and understand the nutritional strategies and trends that athletes use before and during training or competitions. This will identify future clinical trials that can be conducted to identify specific foods that athletes can consume to minimize negative symptoms associated with their consumption and optimize training outcomes.

Bile acids are specific and quantitatively important organic components of bile, which are synthesized by hepatocytes from cholesterol and are involved in the osmotic process that ensures the outflow of bile. Bile acids include many varieties of amphipathic acid steroids. These are molecules that play a major role in the digestion of fats and the intestinal absorption of hydrophobic compounds and are also involved in the regulation of many functions of the liver, cholangiocytes, and extrahepatic tissues, acting essentially as hormones. The biological effects are realized through variable membrane or nuclear receptors. Hepatic synthesis, intestinal modifications, intestinal peristalsis and permeability, and receptor activity can affect the quantitative and qualitative bile acids composition significantly leading to extrahepatic pathologies. The complexity of bile acids receptors and the effects of cross-activations makes interpretation of the results of the studies rather difficult. In spite, this is a very perspective direction for pharmacology.

Objective. Evaluate the cytotoxicity of a new chemical compound «ACVR-IN-01» (in vitro) on a culture of human mesenchymal stromal cells (MSCs). Materials and methods. The characterized MSC culture of the 4th passage from the biobank of cell cultures was used. Test samples «ACVR-IN-01» were prepared at concentrations of 25, 200 and 400 μg/ml. For each tested concentration of «ACVRIN-01» cultivation was carried out in triplicate. A visual assessment of the state of the cell culture was performed after 24, 48 hours and 7 days. The assessment of the functional state of MSCs was carried out by identifying the total number of viable cells and immunophenotypic studies of cell culture. Results. Cultivation of MSCs in a complete growth medium with «ACVR-IN-01» at a concentration of 25, 200 and 400 μg/ml for 7 days does not affect the expression of CD90 and CD105 antigens at all points of observation in vitro. Cultivation of MSCs in a complete growth medium with «ACVR-IN-01» at concentrations of 25, 200 and 400 μg/ml for 7 days did not cause the expression of CD34 and CD45 antigens at all in vitro observation points. Conclusion. The chemical compound «ACVR-IN-01» does not have a significant effect on the linear affiliation of MSCs. Evaluation of the cytotoxicity of the test substance «ACVR-IN-01» (400 μg/ml) on a cell culture in a complete growth medium showed a pronounced decrease in the expression of the CD73 antigen on the 7th day of the experimental in vitro study, accompanied by a loss of the differentiation potential of MSCs in the osteogenic and chondrogenic direction. Key words: herpes virus infection, cytotoxicity, mesenchymal stromal cells, «ACVR-IN-01»