Since its first identification in the United Kingdom in late 2020, the highly
transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant in several countries
raising great concern. We developed a duplex real-time RT-qPCR assay to detect,
discriminate, and quantitate SARS-CoV-2 variants containing one of its mutation
signatures, the ΔHV69/70 deletion, and used it to trace the community circulation
of the B.1.1.7 variant in Spain through the Spanish National SARS-CoV-2 Wastewater
Surveillance System (VATar COVID-19). The B.1.1.7 variant was detected earlier than
clinical epidemiological reporting by the local authorities, first in the southern city
of Málaga (Andalucía) in week 20_52 (year_week), and multiple
introductions during Christmas holidays were inferred in different parts of the country.
Wastewater-based B.1.1.7 tracking showed a good correlation with clinical data and
provided information at the local level. Data from wastewater treatment plants, which
reached B.1.1.7 prevalences higher than 90% for ≥2 consecutive weeks showed that
8.1 ± 2.0 weeks were required for B.1.1.7 to become dominant. The study highlights
the applicability of RT-qPCR-based strategies to track specific mutations of variants of
concern as soon as they are identified by clinical sequencing and their integration into
existing wastewater surveillance programs, as a cost-effective approach to complement
clinical testing during the COVID-19 pandemic.
Wastewater based epidemiology (WBE) offers an overview of the SARS-CoV-2 variants circulating among the population thereby serving as a proper surveillance method.
The variant of concern (VOC) Alpha was first identified in September 2020 in the United Kingdom, and rapidly became dominant across Europe. Our objective was to elucidate the Alpha VOC outcompetition rate and identify mutations in the spike glycoprotein (S) gene, indicative of the circulation of the Alpha VOC and/or other variants in the population through wastewater analysis.
In the period covered by this study (November 2020–April 2021), forteen wastewater treatment plants (WWTPs) were weekly sampled. The total number of SARS-CoV-2 genome copies per L (GC/L) was determined with a Real-Time qPCR, targeting the N gene. Surveillance of the Alpha VOC circulation was ascertained using a duplex RT-qPCR, targeting and discriminating the S gene. Our results showed that in a period of 6 weeks the Alpha VOC was present in all the studied WWTPs, and became dominant in 11 weeks on average. The outcompetition rates of the Alpha VOC were estimated, and their relationship with different parameters statistically analyzed. The rapid spread of the Alpha VOC was influenced by its initial input and by the previous circulation of SARS-COV-2 in the population. This latter point could be explained by its higher transmissibility, particularly advantadgeous when a certain degree of herd immunity exists. Moreover, the presence of signature mutations of SARS-COV-2 variants were established by deep-sequencing of the complete S gene. The circulation of the Alpha VOC in the area under study was confirmed, and additionally two combinations of mutations in the S glycoprotein (T73A and D253N, and S477N and A522S) that could affect antibody binding were identified.
Wastewater-based epidemiology has shown to be an efficient tool to track the circulation of SARS-CoV-2 in communities assisted by wastewater treatment plants (WWTPs). The challenge comes when this approach is employed to help Health authorities in their decision-making. Here, we describe the roadmap for the design and deployment of SARSAIGUA, the Catalan Surveillance Network of SARS-CoV-2 in Sewage. The network monitors, weekly or biweekly, 56 WWTPs evenly distributed across the territory and serving 6 M inhabitants (80% of the Catalan population). Each week, samples from 45 WWTPs are collected, analyzed, results reported to Health authorities, and finally published within less than 72 h in an online dashboard (https://sarsaigua.icra.cat). After 20 months of monitoring (July 20–March 22), the standardized viral load (gene copies/day) in all the WWTPs monitored fairly matched the cumulative number of COVID-19 cases along the successive pandemic waves, showing a good fit with the diagnosed cases in the served municipalities (Spearman Rho = 0.69). Here we describe the roadmap of the design and deployment of SARSAIGUA while providing several open-access tools for the management and visualization of the surveillance data.
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