Objective: The aim of the present study was to assess the concurrent and construct validity of two diet-quality indices, a modified Mediterranean diet score (mMDS) and a Mediterranean-like diet score (MLDS) additionally incorporating unhealthy food choices, as determined by an FFQ. Design: A validation study assessing FFQ intake estimates compared with ten or more unannounced 24 h recalls. Pearson's correlation coefficients, intraclass correlation coefficients (ICC), Bland-Altman plots and the limits of agreement method were used to assess the between-method agreement of scores. Construct validity was shown using associations between nutrient intakes derived from multiple 24 h recalls and the mMDS and MLDS derived from the FFQ. Setting: Gerona, Spain. Subjects: A total of 107 consecutively selected participants from a populationbased cross-sectional survey. Results: Pearson's correlations for the energy-adjusted mMDS and MLDS compared with multiple recalls were 0?48 and 0?62, respectively. The average FFQ energy-adjusted mMDS and MLDS were 102 % and 98 % of the recall-based mMDS and MLDS estimates, respectively. The FFQ under-and overestimated dietary recall estimates of the energy-adjusted MLDS by 28 % and 25 %, respectively, with slightly wider boundaries for the mMDS (31 % and 34 %). The ICC, which assesses absolute agreement, was similar to Pearson's correlations (mMDS 5 0?48 and MLDS 5 0?61). The mean differences between methods were similar across the range of average ratings for both scores, indicating the absence of bias. The FFQderived mMDS and MLDS correlated in the anticipated directions with intakes of eleven (73?3 %) and thirteen of fifteen nutrients (86?7 %), respectively. Conclusions: The FFQ provides valid estimates of diet quality as assessed by the mMDS and MLDS.
During S phase, replication forks can encounter several obstacles that lead to fork stalling, which if persistent 19 might result in fork collapse. To avoid this collapse and to preserve the competence to restart, cells have 20 developed mechanisms that maintain fork stability upon replication stress. In this study, we aimed to 21 understand the mechanisms involved in fork stability maintenance in non-transformed human cells by 22 performing an iPOND-MS analysis in hTERT-RPE cells under different replication stress conditions. Our 23 results show that acute hydroxyurea-induced replication blockade causes the accumulation of large amounts 24 of ssDNA at the fork. Remarkably, this results in the disengagement of replisome components from nascent 25 DNA without compromising fork restart. Notably, CMG helicase maintains its integrity and replisome 26 components remain associated with chromatin upon acute hydroxyurea treatment, whereas replisome stability 27 is lost upon a sustained replication stress that compromises the competence to restart.
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