This study presents on-chip continuous accumulation and concentration of nanoscale samples using a cascade alternating current electroosmosis (cACEO) flow. ACEO can generate flow motion caused by ion movement due to interactions between the AC electric field and the induced charge layer on the electrode surface, with the potential to accumulate particles, especially in low-conductive liquid. However, the intrinsic particle diffusive motion, which is sensitive to particle size, is an essential element influencing accumulation efficiency. In this study, an electrode combining chevron and double-gap geometry embedded in a microfluidic channel was developed to perform efficient three-dimensional (3D) nanoparticle focusing using ACEO. The chevron electrode pattern was introduced upstream of the focusing zone to overcome particle accumulation in scattering zones near the channel sidewall. To demonstrate the efficiency of the proposed device for particle accumulation, three nanoparticle types were used: latex, metal, and biomaterial. Continuous 3D concentration of 50-nm polystyrene particles was confirmed. The concentration factor, determined based on image processing, became quite high when 50-nm gold nanoparticles were used. Moreover, nanoparticles with a 20-nm diameter were accumulated using cACEO. Finally, we used the concentrator chip to accumulate 50-nm liposome particles, confirming that the device could also successfully concentrate biomaterials.
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