Background Mastitis is an inflammation of the mammary glands caused by a microbial infection. The common bacteria causing this infection in dairy farms are Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli. The aptamer is a new biosensor platform for detecting pathogens; however, its use for simultaneous detection of S. aureus, S. agalactiae, and E. coli bacteria has not been reported. This study’s objective is to isolate and characterize polyclonal DNA aptamer with broad reactivity to the mastitis bacteria S. aureus, S. agalactiae, and E. coli using a sequential toggle cell-SELEX. Methods and results The DNA aptamer pool from SELEX 15 was inserted into the pGEM-T easy plasmid. Furthermore, the transformant clones were selected by PCR colony, plasmid isolation, and sequencing. Six DNA aptamers, consisting of S15K3, S15K4, S15K6, S15K13, S15K15, and S15K20 with a constant region and the right size of 81 bp were derived from the sequencing analysis. The secondary structure of the DNA was predicted using Mfold software. The DNA was analyzed with binding characteristics, including binding capacity and affinity (Kd), using qPCR. The results indicated aptamer S15K15 has the highest binding ability into S. agalactiae, while S15K13 performed binding capacity most to E. coli EPEC 4, and S15K3 has the highest capacity of binding to S. aureus BPA-12. Conclusion Aptamer S15K3 has the best binding characteristics on all three bacterial targets.
Mastitis is a complex disease in cattle that it involves interactions between management practices and infectious agents. The common microorganisms causing mastitis are bacteria, besides this disease can be caused by mycoplasma, algae, and yeast. Pathogen microorganisms in milk can be obtained from cattle, human hands, equipment and the environment. This study aims to analyze the metagenomic of pathogen mastitis in cow’s milk from Cicurug, Sukabumi, West Java. ZymoBIOMICSTM DNA Miniprep Kit was used for genome isolation to metagenomic analysis. The 16S rRNA PCR amplification was used for analysis the results of miniprep. Metagenomic analysis from subclinical mastitis milk showed that bacteria in cow’s milk were the genera of Corynebacterium_1 (20.53 %), Corynebacterium (11.67%), Solibacillus (8.78%), Romboutsia (5.45%), Micrococcus (4.18%), Acinetobacter (3.64%), Aerosphaera (1.94%), Ignavigranum (1.90%), Lysinibacillus (1.49%), and Staphylococcus (1.38%).
Pulmonary fibrosis causes scar tissue formation that disrupts the functioning of the lungs. Uncaria gambir (Hunter) Roxb (hereafter gambir)—a plant native to West Sumatra in Indonesia—contains flavonoid (+)-catechin, which has strong antioxidant activity and can be used to combat pulmonary fibrosis. This random in vivo experimental study analyzed the antifibrotic effect of gambir on the lungs of rats with bleomycin-induced fibrosis. The subjects were 10 groups of 10-week-old male rats weighing around 200–250 g. All groups were terminated at the end of the seventh week or on day 50. The lungs were cleaned, and tissues were taken to analyze inflammatory cell counts and TGF-β1 levels using bronchoalveolar lavage (BAL) with ELISA; type I collagen and tissue inhibitor of metalloproteinase 1 (TIMP-1) levels using immunohistochemistry (IHC); and activation of NF-κB using ELISA and Western blot assays. The most severe histopathological characteristic based on the modified Ashcroft score was in the bleomycin group (BG), whereas the mildest was in the 262 mg/kg of the bodyweight antifibrotic gambir-dosed group (AF G262). The results showed a significant difference in the BAL inflammatory cell count ( p = 0.017 ; p < 0.05 ). AF G262 differed most from the other antifibrotic groups in terms of the number of inflammatory cells (0.63), TGF-β1 levels (3.80), and NF-κB levels (0.48), followed by the 131 mg/kg of the bodyweight antifibrotic gambir-dosed group (AF G131), which also differed most from other antifibrotic groups in terms of NF-κB (0.48), TIMP-1 (11.74), and collagen I (14.50) levels. Western blot analysis showed that the fibropreventive and antifibrotic groups had a specific band size of p65, whereas no specific band binding existed in the control group. This study concluded that the administration of AF G262 could improve fibrosis by lysing the extracellular matrix (ECM) in rat lungs.
Gejugan Village is one of the villages in Probolinggo district on the north coast. Gejugan Village is in the west bordering Klaseman Village and Karangpranti Village, Pajarakan District. Gejugan village has local potential that can be developed from their agricultural produce, namely corn. Corn is an alternative food source that contains many nutrients. But the people here still don't have the knowledge to be able to use it. The Socialization Activity for Making SUJU (Corn Milk) to Prevent Stunting and Utilizing Local Potential is expected to provide more knowledge to the Gejugan community in utilizing existing local potential, especially processing corn into corn milk. Besides having good nutrition, corn milk also has more value in the economy. If it is processed properly and properly, this product can be sold in the market. The socialization of making corn milk was attended by 25 residents of Gejugan Village. Material for making corn milk was given by providing materials and hands-on practice during the event. Participants in the socialization of making corn milk enthusiastically listened to and practiced directly making corn milk during the socialization process. From the results of the socialization, the practiced corn milk resulted in 6 bottles of corn milk measuring 360 ml. Participants listened well and the material presented was well received by all participants present. Keywords: Local Potential, Corn Milk, Stunting
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.