First multilocus analysis of the largest Neotropical cichlid genus Crenicichla combining mitochondrial (cytb, ND2, 16S) and nuclear (S7 intron 1) genes and comprising 602 sequences of 169 specimens yields a robust phylogenetic hypothesis. The best marker in the combined analysis is the ND2 gene which contributes throughout the whole range of hierarchical levels in the tree and shows weak effects of saturation at the 3rd codon position. The 16S locus exerts almost no influence on the inferred phylogeny. The nuclear S7 intron 1 resolves mainly deeper nodes. Crenicichla is split into two main clades: (1) Teleocichla, the Crenicichla wallacii group, and the Crenicichla lugubris-Crenicichla saxatilis groups ("the TWLuS clade"); (2) the Crenicichla reticulata group and the Crenicichla lacustris group-Crenicichla macrophthalma ("the RMLa clade"). Our study confirms the monophyly of the C. lacustris species group with very high support. The biogeographic reconstruction of the C. lacustris group using dispersal-vicariance analysis underlines the importance of ancient barriers between the middle and upper Paraná River (the Guaíra Falls) and between the middle and upper Uruguay River (the Moconá Falls). Our phylogeny recovers two endemic species flocks within the C. lacustris group, the Crenicichla missioneira species flock and the herein discovered Crenicichla mandelburgeri species flock from the Uruguay and Paraná/Iguazú Rivers, respectively. We discuss putative sympatric diversification of trophic traits (morphology of jaws and lips, dentition) and propose these species flocks as models for studying sympatric speciation in complex riverine systems. The possible role of hybridization as a mechanism of speciation is mentioned with a recorded example (Crenicichla scottii).

Parallel adaptive radiations have arisen following the colonization of islands by lizards and lakes by fishes. In these classic examples, parallel adaptive radiation is a response to the ecological opportunities afforded by the colonization of novel ecosystems and similar adaptive landscapes that favour the evolution of similar suites of ecomorphs, despite independent evolutionary histories. Here, we demonstrate that parallel adaptive radiations of cichlid fishes arose in South American rivers. Speciation-assembled communities of pike cichlids () have independently diversified into similar suites of novel ecomorphs in the Uruguay and Paraná Rivers, including crevice feeders, periphyton grazers and molluscivores. There were bursts in phenotypic evolution associated with the colonization of each river and the subsequent expansion of morphospace following the evolution of the ecomorphs. These riverine clades demonstrate that characteristics emblematic of textbook parallel adaptive radiations of island- and lake-dwelling assemblages are feasible evolutionary outcomes even in labile ecosystems such as rivers.

El Pescado (literally ‘the fish’) is a lowland stream in the pampean plain, entering the Rio de La Plata at 35˚55¢S,57˚45¢W, 70 km SW of the city of Buenos Aires. The stream develops as a potamon with variable flood frequency along its 36 km length. The water is slightly soft, with relatively high conductivity and dominance of HCO–3 , Na + and Cl – ions. Three fish families, Characidae (29%), Pimelodidae (14.6%) and Loricariidae (12.8%) are most abundant among 55 species. Differences in species composition among four stations with different environmental traits were observed. Flooding slightly affected the fish fauna. In open-water and rocky-bottom stations, both species number and number of individuals were correlated with conductivity, an indication of the decrease of fish density with floods. Species richness was well over worldwide means. Diversity values, higher than in small tropical streams in Venezuela and southern Brazil, ranged from 0.32 to 2.59, being higher during autumn and spring. The stream has a high persistence, with the composition of the fish community maintained or recovering as soon as flooding ends. One Synbranchus marmoratus specimen was found among rocks, the first record of this behaviour for the species.

The Crenicichla mandelburgeri species complex from the Middle Parana ´basin is a diverse group of cichlid species and contains all known ecomorphs found within the entire genus Crenicichla.Here, we study the phylogenetic relationships within the C. mandelburgeri species complex using ddRAD sequencing with focus on its two candidate species flocks endemic to the Iguazu ´and Urugua-ı ´Rivers, and on two putative sympatric species in the Piray Guazu Ŕiver. These species flocks include four and three syntopic species, respectively, which are strongly adapted to different trophic niches and include derived ecomorphs of Crenicichla (molluscivores, a periphyton grazer, and a crevice-feeding thick-lipped invertivore). Our phylogenomic analyses strongly support monophyly and rapid diversification of the Iguazu śpecies flock, but reveal more complex evolutionary histories in the Urugua-ı ´and Piray Guazu ´tributaries. Most species in the Middle Parana ´, including one species in the Urugua-ı ´and both species in the Piray Guazu ´show cytonuclear discordance, and in both of these tributaries, we also found hybridization in one of the resident species. Population-level analyses reveal complete isolation of the Iguazu ´species and coupled with their dramatic ecological diversity, this radiation exemplifies characteristics of a species flock that arose via ecological speciation.

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