Curcuma aeruginosa Roxb. or black turmeric belongs to the family Zingiberaceae is one of the important medicinal plant that is widely distributed in Southeast Asia, including in Indonesia. This plant has been used by the people especially as raw material for medicine and cosmetics industries. However, the development of black turmeric is still constrained by the availability of germplasm collection and improved varieties. The objective of this study was to determine the vegetative morphological characters and phylogenetic relationship among 10 accessions of black turmeric and 3 accessions of genus Curcuma in Indonesia. Data was collected for 15 morphological quantitative characters of black turmeric at the Experimental Garden at Sukamantri IPB based on UPOV and PPVFRA descriptors. The result of principal component analysis has determined three principal components with the proportion of diversity 73.94%. Cluster analysis three clusters accession of black turmeric, namely cluster 1 consisted of accession Cianjur, Malang, Rimbo, and Kendal; cluster 2 consisted of accession Bogor, turmeric (Curcuma longa L.), java turmeric (Curcuma xanthorrhiza Roxb.), Liwa, Natar, white turmeric (Curcuma zedoaria Roscoe.); cluster 3 consisted of accession Cirebon, Kuningan 1, and Kuningan 2. Keywords: cluster, component analysis, germplasm, medicinal plant, phylogenetic
The objective of this study was to determine the efficacy of leptin supplementation into maturation medium on nuclear maturation and fertilization rate of sheep oocytes. The maturation process was conducted using a tissue culture medium (TCM) 199 with four supplementation treatments of leptin namely 0 (control), 10, 50, and 100 ng/mL. Fertilization was conducted in the oocytes supplemented with 10 ng/mL and control using 5×10 6 mL -1 spermatozoa. At the end of maturation and fertilization processes, the oocytes were stained with 2% aceto orcein to determine nuclear maturation rate and pronuclear development. The results showed that the percentage of oocytes reaching metaphase II (MII) stage significantly increased in the oocytes supplemented with leptin at a dose of 10 ng/mL (P<0.05) compared to those supplemented at doses of 50, 100, and 0 ng/mL (93.7±5.9% vs 78.8±4.4%; 72.0±2.6%; 82.1±9.9%). However, fertilization rate of the oocytes supplemented with leptin at a dose of 10 ng/mL and control were similar (72.1±5.5% vs 79.2±7.0%). The data indicated that leptin could improve maturation rate in lower concentration. However, the improved maturation rate of oocytes with leptin supplementation at a dose of 10 ng/mL could not improve the fertilization rate of the oocytes. In conclusion, the supplementation of leptin at a dose of 10 ng/mL could increase the number of oocytes that reached MII stage, but could not increase the fertilization rate.
Study the effect of gamma irradiation on the performance of the plant seed from the chicken's comb (Celosia cristata L.) aims to Obtain mutant plants Celosia unique, interesting, and stable that can be used as a lucrative new varieties on the market. Getting a mutant plant that has the highest diversity in LD50. The experiment was conducted in February 2013 to April 2013. This research was conducted at the Nuclear Energy Agency (BATAN) Pasar Minggu and Development Center of Ornamental Plants Bogor Agricultural Institute (IPB), Taman Kencana, Bogor. This study uses Celosia seeds irradiated with various doses. The dose used is: 0 Gy, 10 Gy, 20 Gy, 30 Gy, 40 Gy, 50 Gy. Observations were made on the Reduction parameter Dose (RD) 50, High Plant (cm), Number Buds (buds), Flower color and Form Flowers. The results showed that the highest dose of 10 plants and 20 gy is 29 cm, while the plant height is lowest at a dose of 40 gy is 24 cm. The highest number of shoots was shown by a dose of 50 gy, gy that radiation dose 10 purplish pink flowers and red. Irradiated interest is soaring upwards but then widened to the side. ©2016 Published by Savana Cendana.
Ginger is one of the demanded spices during pandemic covid-19 which the price rose sharply. Besides the spice utility, Peoples believe that ginger can be used for traditional medicine, improving and preventing folk diseases. Central Kalimantan as consumers of ginger, especially red ginger, is affected by this fluctuation price directly which is indicated by soared price of red ginger almost tripled from IDR 20,000 to more than IDR 55,000. Moreover, the ginger supply of the province seems to depend on other surrounding provinces such as South Kalimantan. This paper analyzes the supply chain and its performance of ginger in Central Kalimantan province, including product, information, and financial flows from the farmers to the consumers. It also evaluates the efficiency and performance of ginger supply chain for improving the balancing system in the future. Data were collected from the farmers in the central of ginger area production, middle traders, traders in the central market in Palangkaraya city, and consumers households. The result shows that Central Kalimantan produce ginger in some regencies such as Pulang Pisau, Kapuas, Kota Waringin, Gunung Mas. However, it did not sufficiently meet ginger’s production but still supported by south Kalimantan province and West Kalimantan province. Therefore, this paper can give the information generally for all drivers, and it can drive appropriate policy for the government, especially for the development plan of ginger in Kalimantan.
Black galingale (Curcuma aeruginosa Roxb.) is an important medicinal plant that is widely distributed in South and South-east Asia; however, its utilities in Indonesia are still limited due to superior varieties and seedling availability. Micro-propagation could provide rapid and true to type seedlings to meet the black galingale demand. The objectives of this study were to develop the best in vitro medium composition for shoot multiplication and to compare the field growth of plants originated from the rhizome and in vitro plantlet. The in vitro experiment was arranged in a randomized complete block design with two factors and five replications. The first factor was BAP concentration consisted of 0, 2, 4, 6, and 8 mg l −1 . The second factor was IAA concentration consisted of 0, 0.5, and 1.0 mg l −1 . Our results showed that number of shoots, number of leaves, and plantlet height were significantly affected by the interaction of IAA and BAP. The highest number of shoots was obtained in the medium containing 6 mg l −1 BAP with no IAA. The in vitro grown plantlets were acclimatized and grown in the field. The growth of these in vitro originated plants was compared with those propagated through the rhizome.
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