Background: Medicinal plants have been known as one of the most important therapeutic agents for cancer treatment and prevention. Capparis spinosa L is a multipurpose plant that contains different bioactive phytochemicals including phenols and flavonoids.Aims: In the present study we explore the invivo antitumor activity of Capparis spinosa L extract and most potent constituents rutin and hesperidin against Ehrlich ascites carcinoma (EAC). Also study the side effect of treatments on different organs (liver, kidney and heart). Material and method: The antitumor effect was assessed by evaluating tumor volume , tumor cell count , survival time and increase in life span of EAC bearing mice. We assessed the effect of Capparis spinosa L extract , rutin and hesperidin on the level of malonaldialdhyde (MDA) and Catalase activity. Also, we estimated their effect on Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) , Albumin , urea , creatinine ,Lactate dehydrogenase activity (LDH), Creatine Kinase-MB activity (CK-MB). Apoptosis was assessed by BCL2 and caspase3 activity. Results : The Capparis spinosa L extract , rutin and hesperidin showed significantly decreased (p<0.001) in the volume of the EAC and in the count of EAC cells and increase the life span of EAC bearing mice. The treatment with Capparis spinosa L extract , rutin and hesperidin showed significantly decreased (p<0.001) the lipid peroxidation marker (MDA) and recovered catalase activity towards normal as compared to positive control. We founded that Capparis spinosa L extract , rutin and hesperidin treatment induced apoptosis demonstrated by an increased in Caspase 3 activity and decreased in BCL2 .The treatment of Capparis spinosa L extract , rutin and hesperidin significantly reduced the elevated levels of ALT , AST ,LDH ,CK-MB,Urea and Creatinine in positive control as compared with negative control. Also, they showed protection for both liver and kidney histopathologically. Conclusions: The present study demonstrated that Capparis spinosa L extract , rutin and hesperidin have potent antitumor activity against Ehrlich ascites carcinoma. The anti-tumor mechanism may be mediated by preventing oxidative damage and induction of apoptosis.
Background: Increasing evidence in both experimental and clinical studies suggests that there is a close link between hyperglycemia, oxidative stress, and diabetic complications. Resveratrol a polyphenolic compound found in various natural food products. Aim: The aim of the present study was to screen insulin sensitization via PPARγ and glucose uptake through activation of PI3K/p-Akt signaling pathway by resveratrol in type 2 diabetic rats Material and methods: Four groups male adult albino rats were used in this study: Negative control, Positive control, Therapeutic group and Standard group. Results: The treatment of resveratrol significantly reduced the elevated levels of blood glucose, Total cholesterol, Triglyceride, LDL-c, Urea and Creatinine in positive control as compared with negative control. Treatment of resveratrol also reverted back the decreased levels of insulin, HDL-c, Irisin, enzymatic antioxidant (catalase and superoxide dismutase) as well as decreased level of the non-enzymatic antioxidant (reduced glutathione) in tissue extracts from different organs of diabetic rats to near normal values. Resveratrol significantly increased expression of the PPAR γ gene in adipose tissue compared to positive control. It also regulated insulin mediated glucose uptake in adipose tissue through activation of PI3K/p-Akt signaling pathway. PPARγ gene showed many different expressions for other side the PPARγ sequences from the adipose tissue confirm there is a mutation with the gene isolated from positive control against therapeutic group, meaning the Type 2 diabetes cause a mutation in the gene sequencing compared to negative control. Hence, we find the opposite with the PPARγ sequencing in therapeutic group. Conclusion: These results clearly suggest that the resveratrol has an antioxidant effects and could improve adipose tissue insulin sensitivity and maintain glucose homeostasis in adipose tissue.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.