In the present study dopamine was measured in the hypothalamus, brainstem, pancreatic islets and plasma, using HPLC. Dopamine D2 receptor changes in the hypothalamus, brainstem and pancreatic islets were studied using [3H] YM-09151-2 in streptozotocin-induced diabetic and insulintreated diabetic rats. There was a significant decrease in dopatnine content in the hypothalamus (P<0.001), brainstem (P<0.001), pancreatic islets (P<0.001) and plasma (P<0.00I) in diabetic rats when compared to control. Scatchard analysis of [3H] YM-09151-2 in the hypothalamus of diabetic rats showed a significant decrease in Bax (P<0.001) and Kd, showing an increased affinity of D2 receptors when compared to control. Insulin treatment did not completely reverse the changes that occurred during diabetes. There was a significant decrease in B,nax (P<0.01) with decreased affinity in the brainstem of diabetic rats. The islet membrane preparation of diabetic rats showed a significant decrease (P<0.001) in the binding of [3H] YM-09 151-2 with decreased Kd (P<0.001) compared to control. The increase in affinity of D2 receptors in hypothalamus and pancreatic islets and the decreased affinity in brainstem were confirmed by competition analysis. Thus our results suggest that the decreased dopamine D, receptor function in the hypothalamus, brainstem and pancreas affects insulin secretion in diabetic rats, which has immense clinical relevance to the management of diabetes.
Trypsin and chymotrypsin inhibitor activities of safflower oilcake were studied before and after irradiation. The various doses to which samples were exposed ranged from 7 Gy to 10 kGy. The trypsin inhibitor is inactivated at 42 Gy, whereas the chymotrypsin inhibitor remains active, even at the much higher dose of 10 kGy. The in vitro digestibility values also showed a significant improvement after irradiation. Exposure to a low dose of 42 Gy is sufficient to improve the nutritional value of the oilcake KEY WORDS: Antinutritional factors, detoxification, digestibility, /n v/tro studies, irradiation, nutritive value, oilseed residues, proteinase inhibitors, safflower cake, unconventional protein.
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