Mango seed kernel oil (MSKO) contains a high concentration of polyphenol and potential as raw materials of food products oil-based and as a natural antioxidant of functional food. Ethanol was used to the extraction of MSKO by response surface methodology (RSM) as a tool to study the optimum extraction conditions of the oil yield and total polyphenol content (TPC). Three-factor-three-level Central Composite Design (CCD) was employed to optimize extraction time (X1), the ethanol concentration (X2), and the ethanol volume (X3) to obtain a high oil yield and TPC. The central points for treatment were 5 h for X1, 86% for X2, and 250 mL for X3. The results showed that the optimum conditions of MSKO extraction were X1=5.18 h, X2=87.84%, and X3=233.43 mL, respectively. Under these conditions, the experimental oil yield and TPC of MSKO were 34.79% and 61.17 mgGAE/g, which was agreed closely to the verification value. The results indicated that MSKO extraction using ethanol could be an effective and advisable method for the large scale production of MSKO extraction.
Rice bran oil (RBO) extraction with ethanol using maceration method accompanied by stirring has been optimized using response surface methodology (RSM) based on central composite design (CCD). Experiments were conducted to investigate the influence of extraction time, ethanol concentration, and ethanol volume on the oil yield, g-oryzanol, and vitamin E of RBO as the response. The experiment consisted of twenty units including six replicates of the center points. The data were analyzed using Design-Expert 10 software to develop and evaluate models and to plot the response curve as 3D surfaces. The result showed that the maximum of the oil yield, g-oryzanol, and vitamin E of RBO was achieved under the optimum conditions of x 1 = 5.30 h, x 2 = 89.21% and x 3 = 686.66 mL (50 g rice bran), respectively. Maximum of the response under these conditions was 14.47%, 783.65 mg.L À1 , and 127.01 mg.L À1 , respectively. This study has resulted in the development of a model for RBO extraction using ethanol as solvent, it is feasible to be applied to the RBO industry with an efficient process, as well as an implementation of the "green" solvent concept.
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