Inflammatory mediators released during seizure act as neuromodulators for nerve cell functioning along with inflammation build-up. They can be targeted for the treatment of epilepsy. Anticonvulsant activity of Viburnum coriaceum extract suggested the potential of the plant in neurological disorders. Preliminary analysis revealed the inhibitory potential of plant root-hexane extract on trypsin and LOX in vitro. This was then fractionated by various chromatographic techniques for the isolation of active compounds. In silico scrutiny of compounds in the GC profile revealed two active compounds, docosane, and methyl palmitate. These compounds were subjected to trypsin and LOX inhibition and their kinetics assays, LOX and COX inhibition assays in LPS induced inflammatory IMR 32 cell lines, and antiepileptic activity on pilocarpine-induced rat models. Docosane showed 50.74% trypsin and 82.51% LOX inhibition. Methyl palmitate showed 91.04% trypsin and 81.12% LOX inhibition. Both the compounds showed a mixed mode of inhibition for trypsin. Docosane showed a mixed-mode and methyl palmitate showed an uncompetitive mode of inhibition for LOX. In the cell culture studies, methyl palmitate had 75.25% and docosane had 67.66% inhibition on COX whereas they had 72.97% and 67.22% inhibition on LOX respectively. Methyl palmitate showed a better antiepileptic activity on rat models.
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