The aim of the present study was to evaluate the effect of injectable platelet-rich fibrin (i-PRF) on cultivated chondrocytes and osteochondral regeneration in critical-sized osteochondral defect of the rabbit's knee in comparison to autologous platelet-rich plasma (PRP). Chondrocytes were first investigated for their ability to proliferate and differentiate in response to PRP and i-PRF. Thereafter, full-thickness critical-sized osteochondral defects 5 mm in diameter and 5 mm in depth were created in the knee joint of 12 adult female New Zealand White rabbits. Defects were regenerated with either PRP or i-PRF and compared to control. Animals were sacrificed at 4 and 12 weeks postoperatively and evaluated histologically by macroscopic and microscopic examination for cartilage regeneration. i-PRF significantly promoted chondrocyte proliferation and mRNA levels of Sox9, collagen type II, and aggrecan when compared to PRP and control. Histological analysis revealed that at 4 weeks, macroscopic ICRS scores from the i-PRF group were significantly enhanced when compared to the PRP and control groups. At 12 weeks post surgery, the microscopic ICRS scores demonstrated that the i-PRF group significantly improved cartilage regeneration when compared to PRP. In conclusion, the use of i-PRF using the low speed centrifugation concept significantly promoted chondrocyte activity and further improved cartilage regeneration when compared to PRP. The histological results revealed early and better cartilage regeneration within 4 weeks postoperatively when i-PRF was utilized and the results were maintained at 12 weeks. Future clinical studies are now needed investigating the regenerative potential of i-PRF in comparison to PRP for knee regeneration.
The aim of the present study was to evaluate treatment of 20 dogs with femur fractures. Twenty (20) dogs with different ages, breeds, and gender admitted to the clinic of
The principles of guided tissue regeneration (GTR) have been used for decades for the treatment of periodontal lesions using barrier membranes to generate new attachment. Guided bone regeneration (GBR) was a principle adopted some years later utilizing a barrier membrane specifically to exclude fast-growing soft tissues from slower-growing alveolar bone. The ideal membrane for GTR and GBR has been defined as having several advantages including being highly biocompatible, wellcontrolled biodegradable, and possessing a space-making ability. While classic barrier membranes were fabricated from non-resorbable materials, more frequently and more widely utilized in the dental field have been biodegradable collagen barrier membranes. These membranes possess the added advantage that they do not require a second surgical intervention to remove the membrane causing the drawbacks of additional patient morbidity, as well as potential tissue damage and wound infection. Resorbable membranes include synthetically fabricated aliphatic polyesters like poly (L-lactide) (PLLA), poly (L-lactide-co-glycolide) (PLGA) and their co-polymers, or natural membranes made of collagen or chitosan. Recently, novel lyophilizing and sterilization procedures have pioneered the development of atelo-collagen type I barrier membranes fabricated from (achilles tendon) bovine sources. The advantages of atelo-collagen (type I) are that it provides complete immunological biocompatibility while having anti-bacterial properties facilitating the regeneration of various tissues found in the oral cavity. Here we investigated this novel bioabsorbable bovine atelo-collagenized membrane (BBAM) (ImploSorb®, Bioimplon, Germany) using scanning electron microscopy (SEM) and evaluated their biocompatibility in a rat gastrocnemius muscle implant model.
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