This study comprise the examination of 100 samples of feed(40), water (36) and litter (24) collected randomly from different chicken shops, houses and farms in Kafr El-Sheikh Governorate. Results of this survey revealed isolation of 14/100(14%) Salmonella species (3)(12.5%) isolates from litter, (5)(13.8%) isolates from water and (6)(25%) isolates from farms. The prevalence rate to Salmonella was (14%) Sensitivity test was made and isolates exhibit resistance against ciprofloxacin and cefotaxime with (89%) and highly sensitivity to amikacin with (100%) so amikacin is the first drug choice for treatment of Salmonella infection. A PCR based assay was developed to developed to detect the prevalence of Salmonella in samples and to evaluate plasmid and chromosome-borne virulence genes (stn-invA) which considered as a target genes for the detection of Salmonella The results of detection of resistance genes (qnr S-blaTEM) as a resistance genes in all isolates of Salmonella.
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