Lucerne is a deep-rooted perennial forage legume with an important role in preventing dryland salinity in southern Australian cropping regions. Annual cereal production has created a water-use imbalance, which is placing the industry under threat through rising saline watertables and resultant dryland salinity. Lucerne is being incorporated into cropping systems to reduce groundwater recharge and improve the sustainability of grain production. Existing lucerne varieties have been developed for the animal industries, primarily for the areas with high rainfall or irrigation. The new challenge is to develop lucernes specifically for southern Australian cropping systems. This paper provides a background literature review of the breeding challenges that are anticipated in the development of these new types of lucerne. Lucerne is intolerant of acidic soils, waterlogging, saline soils, and intensive grazing. Other important attributes covered include the ability of the plant to fix nitrogen with existing rhizobia and be resistant to diseases that affect lucerne and other crops in the rotation. Finally, this paper addresses some of the breeding strategies that will be used to screen lucerne germplasm for tolerances to these soil conditions and diseases.
A preliminary evaluation protocol is described of Medicago sativa that enables a diverse range of germplasm from different winter-activity groups to be evaluated in the same experiment. The protocol was used to compare the seasonal growth patterns of very winter-dormant M. sativa sspp. falcata and caerulea accessions with highly winter-active M. sativa ssp. sativa accessions. Herbage production was strongly related to winter activity in the cumulative first year of growth and in second-year winter months, but completely unrelated in 3 cuts in the following spring. The vertical measurement of plant height was confirmed to be a good indicator of herbage production in winter, but neither stem height nor length were good indicators of spring production. The preliminary evaluation protocol identified highly winter-active class 10–11 germplasm with high winter production and excellent recovery after cutting, and accessions of M. sativa sspp. caerulea and falcata (winter activity class 1) with excellent spring and summer production. These wild relatives of lucerne have great potential to increase the area of cultivated M. sativa in Australia, largely as a component in pasture mixtures. They also offer improved quality traits over winter-active Lucerne, including a higher leaf to stem ratio, and a lower stem thickness and internode length.
A rapid (7 day) solution-based screening test was developed using 15 annual Medicago cultivars and one M. sativa. Based on a relative root regrowth after exposures to aluminium (Al), Zodiac (M. murex), Orion (M. sphaerocarpos) and the M. polymorha cultivars Santiago, Cavalier and Serena had the greatest Al tolerance. Herald (M. littoralis) and Rivoli (M. tornata) were most sensitive. Ranking for Al tolerance from the solution culture correlated well (r = 0.80) with ranking for tolerance of the 16 genotypes grown in an acidic soil (unlimed pHCa 4.1). We screened 17 Australian populations of lucerne (M. sativa) using a 24 h ‘pulse’ of 75 µmol/L Al, and a three day ‘recovery’ of 10 µmol/L Al. We identified and recovered plants with a root regrowth of ≥5 mm in all 17 populations with selection intensities of 2 to 4%. Four of these selected populations (Aurora, UQL-1, A513 and TO2-011) were polycrossed within each population to produce four populations of seed from the cycle 1 selections. The length of root regrowth under Al stress was improved for all four populations of cycle 1 selection (P ≤ 0.001; from 2.6 mm for the original populations to 6.3 mm for the cycle 1 selections). In a subsequent experiment the cycle 2 selections from Aurora, UQL-1 and TO2-011 had significantly greater root regrowth than both the cycle 1 selections (P ≤ 0.001; 8.3 cf. 6.6 mm) and the unselected populations (3.0 mm). The selections from TO2-011 appeared to have greater improvement in the average length of root regrowth after 2 cycles of selection. Selected germplasm was more tolerant than GAAT in our evaluation. Based on estimation of realised heritability, it seemed likely that higher selection intensities would give more rapid improvements in tolerance. Our studies have not investigated the physiological basis of any tolerance of Al which we observed.
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