Chitosan (Chi) is a natural biodegradable cationic polymer with remarkable potency as a vehicle for drug or vaccine delivery. Chi possesses multiple groups, which can be used both for Chi derivatization and for particle formation. The aim of this work was to produce stable nanosized range Chi gels (nanogels, NGs) with different charge and to study the driving forces of complex formation between Chi NGs and proteins or peptides. Positively charged NGs of 150 nm in diameter were prepared from hexanoyl chitosan (HC) by the ionotropic gelation method while negatively charged NGs of 190 nm were obtained from succinoyl Chi (SC) by a Ca 2+ coacervation approach. NGs were loaded with a panel of proteins or peptides with different weights and charges. We show that NGs preferentially formed complexes with oppositely charged molecules, especially peptides, as was demonstrated by gel-electrophoresis, confocal microscopy and HPLC. Complex formation was accompanied by a change in zeta-potential and decrease in size. We concluded that complex formation between Chi NGs and peptide/proteins is mediated mostly by electrostatic interactions.

of heat source evolution during elastic-plastic deformation of titanium alloy Ti-0.8Al-0.8Mn based on contact and non-contact measurements.

Laser shock peening (LSP) is used to enhance surface quality of the metallic structures by the generation of compressive residual stresses on it. This work studies the effect of the main LSP parameters on residual stress fields by the finite-element method. The specimen under investigation is a square plate with a thickness of 3 mm made of Ti-6Al-4V. The performed analysis enhances understanding of LSP application to structures manufactured from this material and this information can be useful for a choice of optimal peening parameters. The effect of the spot size and shape, the pulse energy, the number of peen layers, overlapping of spots and temporal variation of the mechanical pressure induced by plasma is considered and analyzed. A 3D finite-element model based on the Johnson-Cook constitutive relation is developed and verified by the results of residual stress measurements performed for the LSP-treated samples under different conditions. From the obtained results the following main conclusions can be drawn: pulse energy provides the more significant effect although the resulting residual stresses profile tends to some saturation curve; temporal pressure pulse shape and its total duration also substantially alter the residual stress field; the least significant parameter is the spot shape.

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This work is devoted to the experimental investigation of the effect of laser shock peening (LSP) on the thermo-mechanical properties of metals. ARMCO iron was chosen as the model material for the study. Samples were subjected to LSP, and were tested following the procedure of the self-heating (Risitano) technique. To investigate the damage that was induced by heating, the fatigue tests were coupled with infrared thermography measurements. The results of the study showed that the LSP procedure qualitatively changes the temperature evolution in ARMCO iron during cyclic loading. The heating (energy dissipation) of the LSP treated specimen was several times higher than that of the specimen in the initial state. To explain the structural mechanisms of energy dissipation, the microstructure of the specimens was examined using transmission (TEM) and scanning (SEM) electron microscopy, as well as electron backscattering diffraction (EBSD). The results of the structural investigation confirm the qualitative change of defect evolution caused by LSP treatment.