A Seroepidemiological study of Neospora caninum was conducted in Al-Muthana and Al-Nasseria provinces, Iraq on 800 cows serum sample by using commercial Elisa kit. the overall seroprevalence ratio of Neospora caninum was 17.5%, on provincial basis Neospora caninum infection was present in these provinces that was 16 %, 18.4% in Al-Muthana, and Al-Nasseria provinces respectively,which non significant differences between provinces (P<0.05), Comparisons of N.caninum serological status with age groups (5-8 y) showed seropositive rate 21.32% that higher thanother groups with significant differences (P<0.05). antibodies of N.caninum showed in aborted cows 32.29% higher than non aborted cows 7.53% with significant differences (P<0.05). Also the infection rate in dairy cows 19.17% higher than beef cows 12.5% with significant differences (P<0.05).
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