-Germination rate and germination capacity of Eucalyptus globulus Labill. increased significantly with increasing temperature (13º to 33 ºC) for all seed sizes to an optimum at 28 ºC, then decreased. Biggest seeds generally germinated best at all temperatures. Germination was also very sensitive to water potential (0 to -0.75 MPa), with no germination occuring at potentials below -0.25 MPa. Eucalyptus globulus / germination / dimension de la semence / température / potentiel hydrique
Eucalyptus globulus
Two procedures were tested for micropropagation of Ilex aquifolium (English holly), one in which shoots proliferated on solid medium and another one using liquid medium. Different growth regulator treatments and supports were analyzed for optimizing in vitro rooting, showing that indolebutyric acid and agar or cellulose plugs gave the best results. The survival percentage of successfully in vitro or ex vitro rooted plants did not differ significantly between the best treatments. However, the efficiency with ex vitro rooting was 80%, while for in vitro rooting, the final efficiency was 64%. The results show that a correct manipulation of Murashige's stage II of micropropagation and eliminating or decreasing stage III are useful tools to reduce the requirements of acclimatization.
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