strategies to collect dataset efficiently for the S-SAD method, (i) lowering the measurement error using high brilliant short wavelength X-ray, (ii) augmenting the anomalous signal using long wavelength X-ray. To validate these strategies, we experimented on the S-SAD method with standard samples at two synchrotron beamlines, SPring-8/BL41XU and SAGA-LS/BL15. (i)We have collected datasets from standard samples (insulin, thaumatin and lysozyme) using various wavelength between 0.71 to 1.90 Å at the SPring-8 undulator beamline BL41XU. We succeeded in the determination of the phases and building the molecular models of insulin from dataset collected in 0.9 1.9 Å wavelength, thaumatin from 1.5-1.9 Å and lysozyme from 1.5-1.7 Å, respectively. In addition, it ascertained that the dataset collected in longer wavelength allowed the phase determination and model building from lesser redundancy. (ii) SAGA-LS/BL15 has a bending magnet as a light source, and it is able to use X-ray between 0.54 to 5.90 Å wavelengths. The preliminary X-ray diffraction experiment of insulin crystals using 1.5, 2.3, 2.6 and 2.9 Å wavelengths was performed at this beamline. The Rmerge of the collected datasets were about 4-7 % in all wavelengths, and the molecular models could be built automatically from dataset in 1.5 and 2.3 Å wavelengths by S-SAD analysis. From the datasets collected using 2.6 and 2.9 Å wavelengths, we could determine the position of sulfurs and the phase but not build their models automatically due to the less of the resolution.
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