This study aimed to evaluate the effect of addition of aqueous or methanolic Moringa oleifera leaves extract (MOLE) at different levels (0, 100, 200 and 300 µg/ml) as antibiotic alternative to Friesian bull semen extender on sperm characteristics post-dilution, equilibration and thawed. The enzyme activity and antioxidant capacity of bull seminal plasma were determined in post-thawed semen. Five Friesian bulls (3-4 years old and 400-450 kg LBW) were used. Semen was collected from bulls by an artificial vagina twice a week for 5 weeks. After ejaculation semen was evaluated for mass motility and only the ejaculates of ≥70% percentage were pooled and diluted at 37 0 C using citrate-egg yolk extender and divided into seven portions, control (E1), 100 (E2), 200 (E3), 300 (E4) µg aqueous MOLE /ml semen extender or 100 (E5), 200 (E6), 300 (E7) µg methanolic MOLE /ml semen extender. After semen dilution it was equilibrated at 5 0 C for 4 h then it placed in liquid nitrogen at-196 0 C. Frozen semen stored for two month was thawed at 37 0 C for 30 sec. Semen samples were evaluated for characteristics including sperm motility, livability, abnormality, acrosome damage and hypo osmotic swelling test (sperm cells with curled tail) in diluted, equilibrated and thawed semen. Activity of AST, ALT, LDH, catalase and glutathione in post thawed samples seminal plasma were determined. The present results indicated that adding 300 µg of methanolic Moringa oleifera leaves extract to each 1 ml of citrate-egg yolk extender as an alternative to penicillin and streptomycin can improve quality of Friesian bull semen during different preservation stages.
The goal of this study was to see how dietary -glucan (BG) supplementation affected growth performance, blood biochemicals, and antioxidant capacity in growing NZWrabbits. 75 NZW rabbit females, 6 weeks old, with an average live body weight of 845.3735.23 g. Rabbits were distributed into five experimental groups at random (fifteen rabbits in each group). The control group (G1) received only the basal diet, while the G2, G3, G4, and G5 groups received the basal diet supplemented with BG at 0.5,1.0,1.5, and 2.0 g/kg diet, respectively. Growth performance parameters, blood biochemical, and antioxidant enzymes, and immunity markers in the hepatic tissues were determined. Results show that all BG supplements developed (P<0.05) final weight, and gain without any effects on feed efficiency, and viability, and decreased (P<0.05) concentration of glucose, total cholesterol, and triglycerides. The effect of BG on serum activity of ALT and AST was not significant. Dietary BG supplementation increased (P<0.05) SOD, CAT, and GPx levels, and decreased (P<0.05) MDA levels in hepatic tissues. Percentage of NBT and ACP level increased (P<0.05) BG groups (G2-G5) in comparing with G1.The current study concludes that β-glucans represent a promising factor for improving antioxidant capacity, immunity, and lipid profile as well as promoting growth performance of growing rabbits, without adverse effects on liver and kidney function and protein metabolism.
The aim of this study was to evaluate the effect of cryopreservation by conventional straws on viability and normality, in vitro maturation and fertilization and blastocyst production rate of cryopreserved immature buffalo oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes, cryopreserved in tissue culture medium and thawed. Thereafter, oocytes were in vitro matured, fertilized and cultured. The obtained results indicated that viability rate (89.2 vs. 92.7%) and normality percentage (67.7 vs. 80.3%) was lower (P<0.05) for the cryopreserved than fresh oocytes. Percentage of oocytes at stage (in vitro maturation rate) was lower (P<0.05) for cryopreserved than fresh oocytes (40.8 vs. 61.7%). Fertilization rate was lower (P<0.05) for cryopreserved than fresh oocytes (38.5 vs. 75.3%). Blastocyst rate relative to total oocytes was lower (P<0.05) for cryopreserved than fresh oocytes (5.2 vs. 23.6%). This study indicated the possibility of cryopreserved immature buffalo oocytes collected from ovaries of slaughtered buffaloes to in vitro mature, fertilize and develop to embryos at blastocyst stage, but at a little extend of that in fresh case.
he current study aimed to evaluate the role of cumulus-corona radiate complex of immature or in vitro matured bovine oocytes on their vitrification, in vitro fertilization and embryo development. Bovine ovaries were collected from an abattoir and follicular oocytes (3-8 mm in diameter) were aspirated. Oocytes were examined and classified into cumulus oocytes-complexes (COCs), natural denuded oocytes (NDOs) and mechanically denuded oocytes (MDOs). Natural COCs were mechanically denuded by repeated pipetting in phosphate buffer solution (PBS). All types of oocytes were cryopreserved by vitrification as immature or in vitro matured for 24 h prior to vitrification by open-pulled straw cryodevice. After at least 2 weeks of vitrification, all types of vitrified oocytes were evaluated for survival and viability (Normal and abnormal oocytes). Morphologically normal immature oocytes were matured and fertilized in vitro, while morphologically normal mature oocytes were directly fertilized in vitro for determination of cleavage rate (CR). After co-culture of cleaved oocytes for 7 days, rate of morula (MPR) and/or blastocysts (BPR) production was recorded. Results show that survival (SR) and normality (NR) rates were the highest for CCOs (84.47 and 81.73%), followed by MDOs (73.28 and 62.0%), and the lowest for NDOs (60.71 and 54.39%), respectively (P<0.05). SR was higher (P<0.05) for mature than immature oocytes (78.03 vs. 67.60%), while NR was nearly similar for both. CR, MPR and BPR were higher (P<0.05) for CCOs (45.28, 18.05 and 15.28%) than those of MDOs (29.72, 6.06 and 3.03%) and NDOs (25.24, 7.69 and 7.69%), respectively. CR was higher (P<0.05) in mature than in immature oocytes (41.93 vs. 29.36%), while MPR and BPR were nearly similar in both. Based on the obtained results, cumulus cell surrounding cumulus oocytes-complexes play a vital role for survival of bovine oocytes during vitrification and successful in vitro maturation and fertilization as well as embryonic development to morula and blastocyst stages.
his current work was performed to estimate the milk production and composition, blood parameters and some reproductive properties of Egyptian buffalo cows (Bubalus bubalis) fed a digestamin as feed additive. A total of 27 Egyptian buffalo cows were used in this study. The experimental buffaloes aged 4-8 years between the 2nd and 5 th parity at late pregnancy (one month pre partum). Three main experimental basal diets were formulated from corn silage, berseem hay, rice straw and concentrate feed mixture containing 14 % CP and digestsmin was added at 0, 0.1, and 0.5% of DM, respectively. The current study showed that, increasing digestamin supplementation in buffalo cows diets increased significantly (P<0.01) average milk yield, total solids, fat, lactose, protein. On the other hand, there was a significant decrease (P<0.01) in milk total solid not fat, ash and somatic cells count by increasing digestamin supplementation to 0.5% level. Increasing digestamin levels also, significant increased (P<0.01) blood total protein, albumin and total lipids. Oppositely, there was a significant decrease (P<0.01) blood plasma globulin, glucose, AST and ALT concentration with increasing digestamin levels. The experimental diets with digestamin recorded the higher (P<0.01) calf birth, calf birth at weaning, daily weight gain compared to other experimental diets, while the higher body weight and placenta weight of buffalo cows were obtained by control buffalo cows. Generally, there was a significant improved (P<0.01) in reproduction parameters of the experimental buffalo cows by digestamin supplementation. It could be concluded that, digestamin as a feed additive can improve the growth, productive and reproductive performance of Egyptian buffalo cows (Bubalus bubalis). So, it could be used in diets of Egyptian buffalo (Bubalus bubalis) especially with 0.5% of DM.
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