Schwann cell precursors represent a neural crest‐like state with biased multipotency

Kastriti, Maria Eleni; Faure, Louis; Ahsen, Dorothea Von; Bouderlique, Thibault; Boström, Johan; Solovieva, Tatiana; Jackson, Cameron; Bronner‐Fraser, Marianne; Meijer, Dies; Hadjab, Saïda; Lallemend, François; Erickson, Alek; Kaucká, Markéta; Dyachuk, Vyacheslav; Perlmann, Thomas; Lahti, Laura; Křivánek, Jan; Brunet, Jean-François; Fried, Kaj; Adameyko, Igor

doi:10.15252/embj.2021108780

Cited by 38 publications

(50 citation statements)

References 150 publications

(236 reference statements)

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“…The D14 cluster (C7) was characterised by sympathoadrenal/autonomic progenitor markers (GATA3, ASCL1 40,41 ). Cells at D19 consisted of three distinct fractions with mature SCP-like (cluster C8; CRYAB, POSTN, and IGFBP5 42 ), with sympathoblast-like (C11; PHOX2A/B, ELAVL4 16,43 ), or with mesenchymal (C9; COL1A1, FN1) features, in line with findings showing that trunk NC and SCPs are competent to generate mesenchyme 42,44,45 . A fourth cluster (C10) exhibited both sympathoblast and mesenchymal features, suggesting a bridge between cell identities.…”

Section: Differentiation Of In Vitro-derived Human Trunk Nc Cells Rev...supporting

confidence: 83%

A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations

Saldana-Guerrero

Montaño-Gutierrez

Hafemeister

et al. 2022

Preprint

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Early childhood tumours are thought to arise from transformed embryonic cells, which often carry large copy number variants (CNVs). However, it remains unclear how CNVs contribute to embryonic tumourigenesis due to a lack of suitable models. Here we employ human embryonic stem cell (hESC) differentiation to assess the effects of chromosome 17q/1q gains, which are prevalent in the embryonal tumour neuroblastoma (NB). We show that CNVs impair the specification of hESC-derived trunk neural crest (NC) cells and their sympathoadrenal derivatives, the putative cells-of-origin of NB. Overexpression of MYCN, whose amplification co-occurs with CNVs in NB, exacerbates the differentiation block, and enables tumourigenic cell proliferation. We find links between disrupted cell states in vitro and tumour cells and connect these states with stepwise disruption of developmental transcription factor networks. Together, our results chart a possible route to NB and provide a mechanistic framework for the CNV-driven initiation of embryonal tumours.

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Section: Differentiation Of In Vitro-derived Human Trunk Nc Cells Rev...supporting

confidence: 83%

A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations

Saldana-Guerrero

Montaño-Gutierrez

Hafemeister

et al. 2022

Preprint

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“…We found that MITF was present in nerves in what are likely Schwann cell precursors (white arrows in Fig. 8A) as shown by Adameyko's lab (Adameyko et al, 2009;Kastriti et al, 2022). What we observed in our immunostaining was that MITF expression was found along peripheral nerves, but along all its length as acetylated tubulin was, thus it was not complete overlap with acetylated tubulin that stains axons (white arrows in Fig.…”

Section: Labeling Of Trunk Neural Crest Cells and Its Derivativessupporting

confidence: 63%

“…2) Another character is the rapid rostrocaudal progression of TNCC migration and development observed for the Egyptian cobra was the same of the colubrid snake. 3) The presence of MITF in Schwann cell precursors along developing nerves, which suggests that reptile Schwann cell precursors could have the same multipotency as mammalian migrating NCC and thus give rise to its varied derivatives (Kastriti et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Early Development of the Trunk Neural Crest Cells in the Egyptian Cobra Naja h. haje

Khannoon

Alvarado

Poveda

et al. 2022

Preprint

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Trunk neural crest cells (TNCC) are representing a model for epithelial to mesenchymal transition, this correlates the importance of studying the migration of these cells to cancer metastasis. Reptiles are unique group of animals being very morphologically diverse and their close position to synapsid leading to mammals. Recently, more publications focused on the migratory behavior of trunk NCC during embryonic development of squamates. Only one colubrid snake has been studied so far regarding the NCC migration. Here we follow the migratory behavior of TNCC with HNK1 in the elapid snake Naja h. haje from early stage to 16-20 days postoviposition. Our findings show: First, comparing the colubrid snake with the Egyptian cobra shows that both snakes overall follow the conserved TNCC migratory pathways of both birds and mammals by following the rostral and avoiding the caudal portions of the somites. Second, TNCC intra-somitic migration suggests that cobra TNCC could contribute to snake scale formation analogously to turtle shell. Third, the presence of MITF in Schwann cell precursors along developing nerves, which suggests that reptile Schwann cell precursors could have the same multipotency as mammalian migrating NCC and thus give rise to its varied derivatives. Our present results of the Egyptian cobra in combination with those on a colubrid and turtle supports intersomitic TNCC as a unique reptile phenomena.

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“…At the same time, glial and Schwann cell lineage specific markers are upregulated [ 1 ]. Nevertheless, Schwann cell precursors retain a high degree of multipotency, and become primed towards immature Schwann cells or other fates in a gradual manner [ 55 , 62 ].…”

Section: In Vivo Development Of Schwann Cellsmentioning

confidence: 99%

Development and In Vitro Differentiation of Schwann Cells

Hörner

Couturier

Gueiber

et al. 2022

Cells

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Schwann cells are glial cells of the peripheral nervous system. They exist in several subtypes and perform a variety of functions in nerves. Their derivation and culture in vitro are interesting for applications ranging from disease modeling to tissue engineering. Since primary human Schwann cells are challenging to obtain in large quantities, in vitro differentiation from other cell types presents an alternative. Here, we first review the current knowledge on the developmental signaling mechanisms that determine neural crest and Schwann cell differentiation in vivo. Next, an overview of studies on the in vitro differentiation of Schwann cells from multipotent stem cell sources is provided. The molecules frequently used in those protocols and their involvement in the relevant signaling pathways are put into context and discussed. Focusing on hiPSC- and hESC-based studies, different protocols are described and compared, regarding cell sources, differentiation methods, characterization of cells, and protocol efficiency. A brief insight into developments regarding the culture and differentiation of Schwann cells in 3D is given. In summary, this contribution provides an overview of the current resources and methods for the differentiation of Schwann cells, it supports the comparison and refinement of protocols and aids the choice of suitable methods for specific applications.

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Schwann cell precursors represent a neural crest‐like state with biased multipotency

Cited by 38 publications

References 150 publications

A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations

A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations

Early Development of the Trunk Neural Crest Cells in the Egyptian Cobra Naja h. haje

Development and In Vitro Differentiation of Schwann Cells

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