Sarocladium brachiariae sp. nov., an endophytic fungus isolated from Brachiaria brizantha

Liu, Xiaobo

doi:10.5943/mycosphere/8/7/2

Cited by 17 publications

(16 citation statements)

References 3 publications

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“…These findings highlight the common association of Sarocladium species with plants in the Poaceae family, including the rice sheet rot pathogen Sarocladium oryzae and the putative plant-protective endophytes Sarocladium zeae (formerly Acremonium zeae) from maize (Wicklow et al, 2008) and Sarocladium implicatum (formerly Acremonium implicatum) from Brachiaria brizantha (Kelemu et al, 2001). Two Sarocladium species were recently described from collections of grass endophytes, Sarocladium spinificis from Spinifex littoreus, a grass found on the Taiwanese coast (Yeh and Kirschner, 2014), and Sarocladium brachiariae, from B. brizantha grass, collected in China (Liu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential

et al. 2018

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This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited the growth of Bipolaris maydis, Penicillium expansum, and Sclerotinia minor. The results expand our knowledge of the diversity of endophytes associated with tropical grasses and suggest that they may represent new sources of antifungal metabolites for biocontrol and biotechnological purposes.

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Section: Discussionmentioning

confidence: 99%

Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential

et al. 2018

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“…Together with ITS and LSU rDNA sequence phylogenetic analysis, we proposed it as Sarocladium brachiariae (MycoBank no. 814539), a new species of Sarocladium [4]. This new species prominently shows broad-spectrum inhibition to growth of many tested plant pathogenic fungi on solid media plate, including Colletotrichum gloeosporioides of mango trees, Fusarium oxysporium f.sp.…”

Section: Introductionmentioning

confidence: 99%

Genomic characteristics and comparative genomics analysis of the endophytic fungus Sarocladium brachiariae

et al. 2019

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BackgroundSarocladium brachiariae is a newly identified endophytic fungus isolated from Brachiaria brizantha. A previous study indicated that S. brachiariae had antifungal activity; however, limited genomic information restrains further study. Therefore, we sequenced the genome of S. brachiariae and compared it with the genome of S. oryzae to identify differences between a Sarocladium plant pathogen and an endophyte.ResultsIn this study, we reported a gapless genome sequence of a newly identified endophytic fungus Sarocladium brachiariae isolated from Brachiaria brizantha. The genome of S. brachiariae is 31.86 Mb, with a contig N50 of 3.27 Mb and 9903 protein coding genes. Phylogenomic analysis based on single copy orthologous genes provided insights into the evolutionary relationships of S. brachiariae and its closest species was identified as S. oryzae. Comparative genomics analysis revealed that S. brachiaria has 14.9% more plant cell wall degradation related CAZymes to S. oryzae, and 33.3% more fungal cell wall degradation related CAZymes, which could explain the antifungal activity of S. brachiaria. Based on Antibiotics & Secondary Metabolite Analysis Shell (antiSMASH) analysis, we identified a contact helvolic acid biosynthetic gene cluster (BGC) for the first time in S. oryzae. However, S. brachiaria had seven fewer terpene gene clusters, including helvolic acid BGC, compared with S. oryzae and this may be associated with adaptation to an endophytic lifestyle. Synteny analysis of polyketide synthases (PKS), non-ribosomal peptide synthetases (NRPS), and hybrid (PKS-NRPS) gene clusters between S. brachiariae and S. oryzae revealed that just 37.5% of tested clusters have good synteny, while 63.5% have no or poor synteny. This indicated that the S. brachiariae could potentially synthesize a variety of unknown-function secondary metabolites, which may play an important role in adaptation to its endophytic lifestyle and antifungal activity.ConclusionsThe data provided a better understanding of the Sarocladium brachiariae genome. Further comparative genomic analysis provided insight into the genomic basis of its endophytic lifestyle and antifungal activity.

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“…S. oryzae). This diversity is shown by the description of two new species, Sarocladium spinificis, endophytic to coastal grass Spinifex littoreus in Taiwan, and S. brachiariae, recently described in B. brizantha China (Yeh and Kirschner, 2014;Liu et al, 2017). S. implicatum (formerly known as Acremonium implicatum) was identified as a seed-transmitted endophyte of Brachiaria species, where it may play a role in protecting plants against fungal pathogens, such as Drechslera spp., which causes leaf spots (Kelemu et al, 2001).…”

Section: Resultsmentioning

confidence: 99%

Endophytic fungi from Brachiaria grasses in Brazil and preliminary screening of Sclerotinia sclerotiorum antagonists

Gama

Santos

Abreu

et al. 2020

Sci. agric. (Piracicaba, Braz.)

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Fungal endophytes of Brachiaria, a nonhost of Sclerotinia sclerotiorum, may harbor species with antagonistic effects against this plant pathogen. The objective of this work was to investigate the diversity of endophytic fungi associated with different Brachiaria species and hybrids and evaluate their potential to inhibit the plant pathogen S. sclerotiorum. Stem samples from 39 Brachiaria spp. plants were collected in pasture fields and experimental areas of three states of Brazil resulting in 74 endophytes isolated. Twenty-eight species were identified by sequences of the Internal Transcribed Spacer (ITS) and 18S rDNA regions. Paraconiothyrium sp. was the most abundant endophyte, accounting for 24 % (14 isolates) of total, and it was isolated from B. ruziziensis, B. decumbens, B. humidicola, and B. brizantha. Phoma sorghina was the second most abundant taxon, followed by Sarocladium strictum, and Plenodomus sp. In vitro analyses showed that Paraconiothyrium sp., Sarocladium kiliense, Acremonium curvulum, Setophoma terrestris, Dissoconium sp., and Cladosporium flabelliforme exhibited antagonistic activity against S. sclerotiorum, with percentages of growth inhibition ranging from 25 to 60 (p < 0.05). Paraconiothyrium sp. BBXE1 (60 %), BBPB4.1 (60 %), BCMT4.1 (54 %), and S. kiliense (54 %) showed the highest values of Antagonism Percentages (AP). Therefore, fungi with inhibitory activity against S. sclerotiorum such as Paraconiothyrium sp. are naturally endophytic in Brachiaria grasses. Sampling Brachiaria plants Thirty-nine disease-free plants of different species and hybrids of Brachiaria were sampled: B. ruziziensis; B. decumbens cv. Basilisk; B. mutica cv. Angola; hybrid cv. Mulato I and cv. Mulato II; B. humidicola common, cv. Llanero and cv. Tupi; B. brizantha cv. Piatã, cv. Xaraés, and cv. Marandu, in which 11 were collected from pasture fields and 28 from experimental plots. The samples were collected during the rainy and dry seasons from

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Sarocladium brachiariae sp. nov., an endophytic fungus isolated from Brachiaria brizantha

Cited by 17 publications

References 3 publications

Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential

Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential

Genomic characteristics and comparative genomics analysis of the endophytic fungus Sarocladium brachiariae

Endophytic fungi from Brachiaria grasses in Brazil and preliminary screening of Sclerotinia sclerotiorum antagonists

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