Phylogeny of saprobic microfungi from Southern Europe

Hernández-Restrepo, Margarita; Gené, Josepa; Castañeda-Ruíz, Rafael F.; Mena‐Portales, Julio; Crous, Pedro W.; Guarro, Josep

doi:10.1016/j.simyco.2017.05.002

Cited by 123 publications

(176 citation statements)

References 138 publications

(210 reference statements)

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“…Phoma -like asexual morphs have been shown to be scattered within the Pleosporineae, Pleosporales (Chen et al 2017, Valenzuela-Lopez et al 2018). While trying to resolve the natural classification of Phoma -like species in Pleosporales, several new families have been introduced within the sub-order Pleosporineae by various authors (Zhang et al 2009, 2012, Hyde et al 2013, Ariyawansa et al 2015, Hernández-Restrepo et al 2017, Valenzuela-Lopez et al 2018). …”

Section: Introductionmentioning

confidence: 99%

Tzeananiaceae, a new pleosporalean family associated with Ophiocordyceps macroacicularis fruiting bodies in Taiwan

Ariyawansa¹,

Phillips²,

Chuang³

et al. 2018

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The order Pleosporales comprises a miscellaneous group of fungi and is considered to be the largest order of the class Dothideomycetes. The circumscription of Pleosporales has undergone numerous changes in recent years due to the addition of large numbers of families reported from various habitats and with a large amount of morphological variation. Many asexual genera have been reported in Pleosporales and can be either hyphomycetes or coelomycetes. Phoma-like taxa are common and have been shown to be polyphyletic within the order and allied with several sexual genera. During the exploration of biodiversity of pleosporalean fungi in Taiwan, a fungal strain was isolated from mycelium growing on the fruiting body of an Ophiocordyceps species. Fruiting structures that developed on PDA were morphologically similar to Phoma and its relatives in having pycnidial conidiomata with hyaline conidia. The fungus is characterised by holoblastic, cylindrical, aseptate conidiogenous cells and cylindrical, hyaline, aseptate, guttulated, thin-walled conidia. Phylogenetic analysis based on six genes, ITS, LSU, rpb2, SSU, tef1 and tub2, produced a phylogenetic tree with the newly generated sequences grouping in a distinct clade separate from all of the known families. Therefore, a new pleosporalean family Tzeananiaceae is established to accommodate the monotypic genus Tzeanania and the species T.taiwanensis in Pleosporales, Dothideomycetes. The Ophiocordyceps species was identified as O.macroacicularis and this is a new record in Taiwan.

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Section: Introductionmentioning

confidence: 99%

Tzeananiaceae, a new pleosporalean family associated with Ophiocordyceps macroacicularis fruiting bodies in Taiwan

Ariyawansa¹,

Phillips²,

Chuang³

et al. 2018

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“…Dendryphiella can be differentiated by the presence of nodulose conidiophores and conidiogeneous cells with pores surrounded by a thickened and darkened wall, which are absent in Neodrendryphiella . Other genera of the Dothideomycetes, although accommodated in different orders or families with a similar conidiogenous apparatus are Dendryphion (Toluraceae, Pleosporales) (Crous et al 2014, Crous et al 2015), Dendryphiopsis (Kirschsteiniotheliaceae, Kirschsteiniotheliales) (Su et al 2016, Hernández-Restrepo et al 2017) and Paradendryphiella (Pleosporaceae, Pleosporales) (Woudenberg et al 2013). However, the genus Diplococcium in Leotiomycetes also shows similar asexual propagules (Shenoy et al 2010, Hernández-Restrepo et al 2017), which complicates the classification of these fungi based exclusively on morphological features.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, they were closely related to the strain CBS 139.95 of Diplococcium (Di.) asperum, which was proven to be related to the Dictyosporiaceae (Shenoy et al 2010, Boonmee et al 2016). It is well known that the genus Diplococcium is highly polyphyletic, with species distributed across different classes of the Ascomycota, with its type species, Di.spicatum , being related to the Helotiales in Leotiomycetes (Shenoy et al 2010, Hernández-Restrepo et al 2017). …”

Section: Introductionmentioning

confidence: 99%

Neodendryphiella, a novel genus of the Dictyosporiaceae (Pleosporales)

Iturrieta-González¹,

Gené²,

Guarro³

et al. 2018

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In a survey of soil and herbivore dung microfungi in Mexico and Spain, several dendryphiella-like species were found. Phylogenetic analyses based on ITS and LSU sequences showed that these fungi belonged to the family Dictyosporiaceae (Pleosporales) and represent an undescribed monophyletic lineage distant from Dendryphiella. Therefore, the genus Neodendryphiella is proposed to accommodate three new species, N.mali, N.michoacanensis and N.tarraconensis. The novel genus shares morphological features with Dendryphiella such as differentiated conidiophores and polytretic integrated conidiogenous cells, that produce acropetal branched chains of conidia. Neodendryphiella differs in the absence of nodulose conidiophores bearing conidiogenous cells with pores surrounded by a thickened and darkened wall, typical features in the conidiogenous apparatus of Dendryphiella. In addition, the phylogenetic and morphological analysis of several reference strains of different Dendryphiella species, available for comparison, support the proposal of D.variabilissp. nov., which mainly differs from the other species of the genus by having conidia up to 7 septa and highlight that D.vinosa and D.infuscans are obscure species that require further taxonomic review.

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“…Single conidial isolates were established from sporulating conidiomata in Petri dishes containing water agar (WA; Difco agar 5 g, tap water 1 l). Colonies were sub-cultured on potato carrot agar (PCA; potatoes 20 g; carrots 20 g; agar 20 g; distilled water 1 l) and oatmeal agar (OA; oatmeal 30 g; agar 18 g; distilled water 1 l) as described previously (Hernández-Restrepo et al 2017). Herbarium type material and the ex-type strain are maintained at the BIOTEC Bangkok herbarium (BBH) and at the BIOTEC culture collection (BCC; both Pathum Thani, Thailand), respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Micromorphological descriptions and measurements for 30 replicates of relevant features were carried out from mature conidiomata and conidia mounted in lactic acid 90%. Photomicrographs were made following Hernández-Restrepo et al (2017).…”

Section: Methodsmentioning

confidence: 99%

New nematicidal and antimicrobial secondary metabolites from a new species in the new genus, Pseudobambusicola thailandica

Rupcic¹,

Hernández-Restrepo²,

Crous³

et al. 2018

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During the course of a study on the functional biodiversity of the mycobiota inhabiting rainforests in Thailand, a fungal strain was isolated from a plant sample and shown to represent an undescribed species, as inferred from a combination of morphological and molecular phylogenetic methods. Molecular phylogenetic analyses, based on four DNA loci, revealed a phylogenetic tree with the newly generated sequences clustering in a separate branch, together with members of the Sulcatisporaceae (Pleosporales, Ascomycota). The Thai specimen morphologically resembled Neobambusicola strelitziae in having pycnidial conidiomata with phialidic conidiogenous cells that produce both fusoid-ellipsoid macroconidia and subcylindrical microconidia. However, the new fungus, for which the name Pseudobambusicola thailandica is proposed, differs from N. strelitziae in having conidiomata with well-defined necks, the presence of globose to subglobose thick-walled cells adjacent to conidiomata and the production of chlamydospores in culture. When cultures of P. thailandica, growing on water agar, were confronted with Caenorhabditis elegans nematodes, worms approaching the fungal mycelia were killed. This observation gave rise to a study of its secondary metabolites and six novel and two known compounds were isolated from submerged cultures of P. thailandica. The structures of metabolites 1–6, for which the trivial names thailanones A–F are proposed, were elucidated using a combination of spectral methods, including extensive 1 and 2D NMR analysis and high resolution mass spectrometry. Compounds 4 and 8 showed strong nematicidal and weak antifungal activity, whereas all other tested compounds showed moderate to weak nematicidal activity but no significant effects in the serial dilution assay against various fungi and bacteria. Compounds 1 and 8 also inhibited growth of the pathogenic basidiomycete Phellinus tremulae in a plate diffusion assay.

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Phylogeny of saprobic microfungi from Southern Europe

Cited by 123 publications

References 138 publications

Tzeananiaceae, a new pleosporalean family associated with Ophiocordyceps macroacicularis fruiting bodies in Taiwan

Tzeananiaceae, a new pleosporalean family associated with Ophiocordyceps macroacicularis fruiting bodies in Taiwan

Neodendryphiella, a novel genus of the Dictyosporiaceae (Pleosporales)

New nematicidal and antimicrobial secondary metabolites from a new species in the new genus, Pseudobambusicola thailandica

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