Metabolites from nematophagous fungi and nematicidal natural products from fungi as an alternative for biological control. Part I: metabolites from nematophagous ascomycetes

Degenkolb, Thomas; Vilcinskas, Andreas

doi:10.1007/s00253-015-7233-6

Cited by 125 publications

(75 citation statements)

References 92 publications

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“…Although, many studies have tried to isolate and utilize nematode-trapping fungi as biocontrol agents for plant-feeding nematodes, they have yet to be utilized commercially (Degenkolb and Vilcinskas, 2016). This may be because the fungi that have thus far been worked with have been selected mainly on their ability to be readily cultured which is clearly an important determinant for any potential marketable biocontrol agent.…”

Section: Discussionmentioning

confidence: 99%

Detection of Invertebrate Suppressive Soils, and Identification of a Possible Biological Control Agent for Meloidogyne Nematodes Using High Resolution Rhizosphere Microbial Community Analysis

et al. 2016

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White clover (Trifolium repens) is the key legume component of New Zealand pastoral agriculture due to the high quality feed and nitrogen inputs it provides. Invertebrate pests constrain white clover growth and this study investigated rhizosphere-associated fungal controls for two of these pests and attempts to disentangle the underpinning mechanisms. The degree of suppressiveness of 10 soils, in a latitudinal gradient down New Zealand, to added Meloidogyne hapla and Costelytra zealandica scarab larvae was measured in untreated soil. Most of the soils showed no suppressive activity against these pests but two showed activity against M. hapla and two against C. zealandica. Rhizosphere fungi responsible for pest suppressive responses were elucidated via next-generation sequencing. In the M. hapla-suppressive soils nematode-trapping Orbiliomycetes fungi were present in significantly greater abundance than non-suppressive soils and their abundance increased further with addition of M. hapla. A comparison of plant growth and the rhizosphere fungal community between untreated and irradiated soil was carried out on 5 of the 10 soils using Pyronota as the scarab larvae. Soil irradiation either: reduced (by 60–70%); increased (16×) or made no difference to white clover growth across the five soils tested, illustrating the range of microbial impacts on plant production. In one of the M. hapla suppressive soils irradiation resulted in a significant increase in nematode galling suggesting that Orbiliomycetes fungi were indeed responsible for the suppressive effect. Lack of consistent changes in soil macronutrients and pH post-irradiation suggest these were not responsible for plant or invertebrate responses. The use of next generation sequencing in controlled pot trials has allowed identification of a potential biological control organism and bioindicator for M. hapla suppression.

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Section: Discussionmentioning

confidence: 99%

Detection of Invertebrate Suppressive Soils, and Identification of a Possible Biological Control Agent for Meloidogyne Nematodes Using High Resolution Rhizosphere Microbial Community Analysis

et al. 2016

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“…Nematodes are a major constraint to successful vegetable production all over the world, causing severe damage that leads to yield losses. Plant-parasitic nematodes are estimated to cause global annual losses of more than US$ 100 billion (Chitwood, 2003;Nicol et al 2011;Degenkolb and Vilcinskas, 2016). Nematode damage in crops is non-specific and causes a range of symptoms from mild to severe, such as wilting, stunting, reduced vigor, nutrient deficiency, root lesions, reduced flowering, fruit loss, poor yield, and even death.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Root-Knot Nematode Meloidogyne incognita by Chitinolytic Trichoderma spp.

Sayed

Abdelrahman

Ragab

et al. 2019

Egyptian Journal of Agronematology

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The inhibitory potential of chitinolytic Trichoderma spp. was assayed against Meloidogyne incognita in vitro and in vivo. Sixteen Trichoderma isolates were assayed for chitinase activity using basal solid medium supplemented with colloidal chitin. Isolates recorded high chitinolytic activity were identified morphologically as Trichoderma asperelloides, T. hamatum, T. harzianum and T. viride. The four species were quantitatively assayed for total and specific chitinase activity. T. asperelloides showed maximum chitinase activity (1.736 U/mL). In vitro studies revealed that egg hatching and second juveniles vitality of root-knot nematodes were significantly inhibited by chitinase-containing growth culture of the four selected chitinolytic Trichoderma species. However, the reduction effect was directly correlated with the concentration of fungal growth suspension. Under greenhouse conditions, three sequentially treatments of soil with homogenized culture suspension were studied. Trichoderma species significantly reduced the final nematode population by 97.81% and root galling by 92.81%. Moreover, all tested Trichoderma species obviously promoted tomato growth compared to untreated control. Significant positive correlation between the biocontrol efficacy of Trichoderma species against root-knot nematodes and their chitinolytic activity was confirmed. The most potential chitinolytic species, T. asperelloides, also recorded the highest biocontrol activity against M. incognita.

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“…Thus, understanding the chemical basis for fungi nematode interactions offers natural biocontrol strategies (Anke et al 1995). According to Degenkolb and Vilcinskas (2016), approximately 700 species of nematophagous fungi have been described so far and four ecophysiological categories have been proposed. However, little has been done to screen for metabolites in nematophagous fungi or, for that measure, nematicidal metabolites in other fungi since the first studies of this kind during the 1990s (Stadler et al 1993a, b, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…Highly specific, preferably soil-borne antagonists are best suited for this purpose (Degenkolb and Vilcinskas 2016). …”

Section: Introductionmentioning

confidence: 99%

New nematicidal and antimicrobial secondary metabolites from a new species in the new genus, Pseudobambusicola thailandica

Rupcic¹,

Hernández-Restrepo²,

Crous³

et al. 2018

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During the course of a study on the functional biodiversity of the mycobiota inhabiting rainforests in Thailand, a fungal strain was isolated from a plant sample and shown to represent an undescribed species, as inferred from a combination of morphological and molecular phylogenetic methods. Molecular phylogenetic analyses, based on four DNA loci, revealed a phylogenetic tree with the newly generated sequences clustering in a separate branch, together with members of the Sulcatisporaceae (Pleosporales, Ascomycota). The Thai specimen morphologically resembled Neobambusicola strelitziae in having pycnidial conidiomata with phialidic conidiogenous cells that produce both fusoid-ellipsoid macroconidia and subcylindrical microconidia. However, the new fungus, for which the name Pseudobambusicola thailandica is proposed, differs from N. strelitziae in having conidiomata with well-defined necks, the presence of globose to subglobose thick-walled cells adjacent to conidiomata and the production of chlamydospores in culture. When cultures of P. thailandica, growing on water agar, were confronted with Caenorhabditis elegans nematodes, worms approaching the fungal mycelia were killed. This observation gave rise to a study of its secondary metabolites and six novel and two known compounds were isolated from submerged cultures of P. thailandica. The structures of metabolites 1–6, for which the trivial names thailanones A–F are proposed, were elucidated using a combination of spectral methods, including extensive 1 and 2D NMR analysis and high resolution mass spectrometry. Compounds 4 and 8 showed strong nematicidal and weak antifungal activity, whereas all other tested compounds showed moderate to weak nematicidal activity but no significant effects in the serial dilution assay against various fungi and bacteria. Compounds 1 and 8 also inhibited growth of the pathogenic basidiomycete Phellinus tremulae in a plate diffusion assay.

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Metabolites from nematophagous fungi and nematicidal natural products from fungi as an alternative for biological control. Part I: metabolites from nematophagous ascomycetes

Cited by 125 publications

References 92 publications

Detection of Invertebrate Suppressive Soils, and Identification of a Possible Biological Control Agent for Meloidogyne Nematodes Using High Resolution Rhizosphere Microbial Community Analysis

Detection of Invertebrate Suppressive Soils, and Identification of a Possible Biological Control Agent for Meloidogyne Nematodes Using High Resolution Rhizosphere Microbial Community Analysis

Biocontrol of Root-Knot Nematode Meloidogyne incognita by Chitinolytic Trichoderma spp.

New nematicidal and antimicrobial secondary metabolites from a new species in the new genus, Pseudobambusicola thailandica

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