Blastobotrys (Arxula) adeninivorans: a promising alternative yeast for biotechnology and basic research

Abstract

Blastobotrys adeninivorans (syn. Arxula adeninivorans) is a non-conventional, non-pathogenic, imperfect, haploid yeast, belonging to the subphylum Saccharomycotina, which has to date received comparatively little attention from researchers. It possesses unusual properties such as thermo- and osmotolerance, and a broad substrate spectrum. Depending on the cultivation temperature B. (A.) adeninivorans exhibits different morphological forms and various post-translational modifications and protein expression properties that are strongly correlated with the morphology. The genome has been completely sequenced and, in addition, there is a well-developed transformation/expression platform, which makes rapid, simple gene manipulations possible. This yeast species is a very good host for homologous and heterologous gene expression and is also a useful gene donor. Blastobotrys (A.) adeninivorans is able to use a very wide range of substrates as carbon and/or nitrogen sources and is an interesting organism owing to the presence of many metabolic pathways, for example degradation of n-butanol, purines and tannin. In addition, its unusual properties and robustness make it a useful bio-component for whole cell biosensors. There are currently a number of products on the market produced by B. (A.) adeninivorans and further investigation may contribute further innovative solutions for current challenges that exist in the biotechnology industry. Additionally it may become a useful alternative to existing commercial yeast strains and as a model organism in research. In this review we present information relevant to the exploitation of B. (A.) adeninivorans in research and industrial settings. Copyright © 2016 John Wiley & Sons, Ltd.

Production and characterization of yeasts grown on media composed of spruce-derived sugars and protein hydrolysates from chicken by-products

“…B. adeninivorans is a yeast with a very broad substrate spectrum, utilizing, apart from monosaccharides, also for instance aromatic compounds, and degrading phytate. It is also osmo-and thermotolerant and therefore promising for industrial applications [30].…”

“…1 shows very low growth of Thermosacc ® Dry and B. adeninivorans on the urea containing medium, indicating that these yeasts lack the enzyme apparatus for urea assimilation. We considered B. adeninivorans interesting as it can use a large variety of substrates as a carbon and nitrogen source [30,31]. These nitrogen sources include purines, which are abundant in chicken by-products [32].…”

Characterization of a Maltase from an Early-Diverged Non-Conventional Yeast Blastobotrys adeninivorans

“…adeninivorans has several biotechnologically relevant properties: accumulation of lipids [6], salt tolerance, temperature-induced filamentation that promotes protein secretion and the ability to use a wide range of carbon and nitrogen sources, including purines, tannin and butanol, that are unusual nutrients for yeasts [2,7]. B. adeninivorans has been engineered for butanol production, applied in kits for the detection of hormones and dioxine in water as well as for manufacturing of tannase and cutinases [7]. Some other enzymes of B. adeninivorans have also been investigated, including alcohol dehydrogenase [8], extracellular glucoamylase [9] and invertase [10].…”

Agdc1p – a Gallic Acid Decarboxylase Involved in the Degradation of Tannic Acid in the Yeast Blastobotrys (Arxula) adeninivorans

“…The non-conventional, non-pathogenic, imperfect, dimorphic yeast Arxula adeninivorans (syn. Blastobotrys adeninivorans ) is a fungal organism with great biotechnological potential due to its wide substrate spectrum and robustness against osmotic, salt and temperature stresses (Middelhoven et al, 1984 ; Wartmann et al, 1995 ; Malak et al, 2016 ). It grows in harsh environments, tolerates up to 20% NaCl in the medium, endures temperatures of ≤48°C.…”