Advances and perspectives in the generation of polyploid plant species

Tamayo‐Ordóñez, M. C.; Espinosa-Barrera, Laura; Tamayo-Ordóñez, Yahaira; Ayil‐Gutiérrez, Benjamín Abraham; Teyer, Lorenzo Felipe Sánchez

doi:10.1007/s10681-016-1646-x

Cited by 32 publications

(25 citation statements)

References 216 publications

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“…The genomic redundancy created by polyploidy allows relaxed selective constraints and functional divergence of gene copies, which can generate new phenotypes in the long-term evolutionary process (Adams and Wendel, 2005;Comai, 2005). Immediate phenotypic effects of polyploidy are also observed compared to their diploid progenitors, such as increased cell and organ size, changes in flowering time, and greater vigor and biomass (Osborn et al, 2003;Tamayo-Ordóñez et al, 2016). The molecular mechanisms contributing to phenotypic variation shortly after polyploidization are not well-understood, but probably involve more complex genetic and epigenetic effects of higher allelic dosage and heterosis (Osborn et al, 2003;Jackson and Chen, 2010;Renny-Byfield and Wendel, 2014;Fort et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Insights Into the Genetic Basis of Blueberry Fruit-Related Traits Using Diploid and Polyploid Models in a GWAS Context

et al. 2018

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Polyploidization is an ancient and recurrent process in plant evolution, impacting the diversification of natural populations and plant breeding strategies. Polyploidization occurs in many important crops; however, its effects on inheritance of many agronomic traits are still poorly understood compared with diploid species. Higher levels of allelic dosage or more complex interactions between alleles could affect the phenotype expression. Hence, the present study aimed to dissect the genetic basis of fruit-related traits in autotetraploid blueberries and identify candidate genes affecting phenotypic variation. We performed a genome-wide association study (GWAS) assuming diploid and tetraploid inheritance, encompassing distinct models of gene action (additive, general, different orders of allelic interaction, and the corresponding diploidized models). A total of 1,575 southern highbush blueberry individuals from a breeding population of 117 full-sib families were genotyped using sequence capture and next-generation sequencing, and evaluated for eight fruit-related traits. For the diploid allele calling, 77,496 SNPs were detected; while 80,591 SNPs were obtained in tetraploid, with a high degree of overlap (95%) between them. A linear mixed model that accounted for population and family structure was used for the GWAS analyses. By modeling tetraploid genotypes, we detected 15 SNPs significantly associated with five fruit-related traits. Alternatively, seven significant SNPs were detected for only two traits using diploid genotypes, with two SNPs overlapping with the tetraploid scenario. Our results showed that the importance of tetraploid models varied by trait and that the use of diploid models has hindered the detection of SNP-trait associations and, consequently, the genetic architecture of some commercially important traits in autotetraploid species. Furthermore, 14 SNPs co-localized with candidate genes, five of which lead to non-synonymous amino acid changes. The potential functional significance of these SNPs is discussed.

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Section: Introductionmentioning

confidence: 99%

“…Polyploids exhibiting new phenotypic traits can outperform their diploid counterparts, occupy new niches, and become ecologically and agriculturally important (Tamayo-Ordóñez et al, 2016;Spoelhof et al, 2017). Many important crops are polyploids with varied ploidy levels and mode of origin (i.e., autoor allopolyploids).…”

Section: Introductionmentioning

confidence: 99%

Insights Into the Genetic Basis of Blueberry Fruit-Related Traits Using Diploid and Polyploid Models in a GWAS Context

et al. 2018

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“…Allopolyploids are the products of merging two or more genomes by intraspecific or interspecific hybridisation [6,7]. Allopolyploid breeding has been applied successfully in forestry due to its advantages of high biomass and fitness.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Tetraploid Induction from Leaf and Petiole Explants of Hybrid Sweetgum (Liquidambar styraciflua × Liquidambar formosana)

Yan

ZeWei

et al. 2017

Forests

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Abstract:Liquidambar is an important forestry species used to generate many commercial wood products, such as plywood. Inducing artificial polyploidy is an effective method to encourage genetic enhancements in forestry breeding. This report presents the first in vitro protocol for the induction of genus Liquidambar tetraploids based on the established in vitro regeneration system of hybrid sweetgum (Liquidambar styraciflua × Liquidambar formosana). The leaves and petioles from three genotypes were pre-cultured in woody plant medium (WPM) supplemented with 0.1 mg/L thidiazuron (TDZ), 0.8 mg/L benzyladenine (BA), and 0.1 mg/L α-naphthalene acetic acid (NAA) for a variable number of days (4, 6 or 8 days), and exposed to varying concentrations of colchicine (120, 160, 200 mg/L) for 3, 4 or 5 days; the four factors were investigated using an orthogonal experimental design. Adventitious shoots were rooted in 1/2 WPM medium supplemented with 2.0 mg/L indole butyric acid (IBA) and 0.1 mg/L NAA. The ploidy level was assessed using flow cytometry and chromosome counting. Four tetraploids and nine mixoploids were obtained from the leaves. Pre-treatment of the leaves for 8 days and exposure to 200 mg/L colchicine for 3 days led to the most efficient tetraploid induction. Producing 11 tetraploids and five mixoploids from petioles, the best tetraploid induction treatment for petioles was almost the same as that with the leaves, except that pre-culturing was required for only 6 days. In total, 15 tetraploids were obtained with these treatments. This study described a technique for the induction of tetraploid sweetgum from the leaves or petioles of parental material. Based on the success of polyploid breeding in other tree species, the production of hybrid sweetgum allotetraploids constitutes a promising strategy for the promotion of future forestry breeding.

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“…Stomatal conductance is a function of the density, size, and opening of stomata present in each plant species . In the genus Agave , groups of 3–5 stomata are arranged in short rows and it is known that stomatal densities vary according to ploidy level of accessions; a phenomenon know as “gigas.” Comparing different ploidy level accessions from the same species of Agave , those with higher ploidy level ( A. angustifolia Haw. “Chelem ki” 2 n = 6 x = 180; and A. fourcroydes Lem.…”

Section: Drought and High‐temperature Avoidance Of Agave Lmentioning

confidence: 99%

“…The American plant genus Agave L. includes over 200 species, of which 166 are distributed throughout México . The crassulacean acid metabolism (CAM), superficial root system, and succulent leaves with thick, waxy cuticle of the species of Agave provides them with adaptive advantage in an ample range of geographical zones, as a result of their resistance to abiotic and biotic environmental factors including drought, salinity, extreme temperatures, and pathogens . These genetic, morphological, and physiological skills of economically important crops in the genus Agave make its species promising for minimizing the risk of losses as a result of the global climate change in present and future biotechnological applications .…”

Section: Introductionmentioning

confidence: 99%

Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest in Agave L. for genetic improvement and biocatalysis

Tamayo‐Ordóñez

Ayil‐Gutiérrez

Tamayo-Ordóñez

et al. 2018

Biotechnology Progress

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Several of the over 200 known species of Agave L. are currently used for production of distilled beverages and biopolymers. The plants live in a wide range of stressful environments as a result of their resistance to abiotic stress (drought, salinity, and extreme temperature) and pathogens, which gives the genus potential for germplasm conservation and biotechnological applications that may minimize economic losses as a result of the global climate change. However, the limited knowledge in the genus of genome structure and organization hampers development of potential improved biotechnological applications by means of genetic manipulation and biocatalysis. We reviewed Agave and plant sequences in the GenBank NCBI database for identifying genes with biotechnological potential for fermentation, bioenergy, fiber improvement, and in vivo plant biopolymer production. Three-dimensional modeling of enzyme structures in plant accessions revealed structural differences in sucrose 1-fructosyltransferase, fructan 1-fructosyltransferase, fructan exohydrolase (1-FEH), cellulose synthase (CES), and glucanases (EGases) with possible effects in fructan, sugar, and biopolymer production. Although the coding genes of FEH and enzymes involved in biopolymer production (CES, sucrose synthase, and EGases) remain unidentified in Agave L., our results could aid isolation of such genes in Agave. By comparing nucleotide and amino acid sequences in accessions of Agave and other plants, knowledge may be gained about transcriptional regulation and enzymatic activity factors. Future study is needed of biotechnological application of Agave genes for crop breeding aided by genetic engineering and biocatalysis.

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Advances and perspectives in the generation of polyploid plant species

Cited by 32 publications

References 216 publications

Insights Into the Genetic Basis of Blueberry Fruit-Related Traits Using Diploid and Polyploid Models in a GWAS Context

Insights Into the Genetic Basis of Blueberry Fruit-Related Traits Using Diploid and Polyploid Models in a GWAS Context

In Vitro Tetraploid Induction from Leaf and Petiole Explants of Hybrid Sweetgum (Liquidambar styraciflua × Liquidambar formosana)

Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest in Agave L. for genetic improvement and biocatalysis

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