A new substance to relax polychaete worms (Annelida) prior to morphological study

Rahimian, Hassan; Glasby, Christopher J.

doi:10.3897/zookeys.594.8061

Cited by 8 publications

(3 citation statements)

References 20 publications

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“…Lpt/Wp was obtained from illustrations directly (excepting Namalycastis geayi, N. longicirris and N. siolii). This measure was preferred over the number of chaetigers reached when posterodorsal tentacular cirri are placed backwards (Glasby 1999), because differences in preparation of specimens and contraction of body during fixation can cause misleading measurements (Oliveira et al 2010, Bonyadi-Naeini et al 2016.…”

Section: Methodsmentioning

confidence: 99%

The troglomorphic adaptations of Namanereidinae (Annelida, Nereididae) revisited, including a redescription of Namanereis cavernicola (Solís-Weiss & Espinasa, 1991), and a new Caribbean species of Namanereis Chamberlin, 1919

Conde-Vela¹

2017

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Most species belonging to Namanereis Chamberlin, 1919 live in freshwater and subterranean waters, even in water bodies several meters above sea level. A new species belonging to the stygobiont Namanereis group is described here; it shares the common morphological characters of absence of eyes and pigmentation, bifid jaws, elongation of chaetae and cirri, which have been recently regarded as troglomorphies. Because these features are used in evaluations of phylogenetic affinity in Namanereis, a review of these features was made for all known namanereidins, and it was extended to include species in Namalycastis Hartman, 1959. It is shown that elongation of tentacular and dorsal cirri, or elongation of upper sub-acicular falcigers in pre-or post-acicular fascicles, are not exclusive or restricted to species living in subterranean habitats or to Namanereis, because these features are also present in several Namalycastis species. However, the presence of bifid jaws, and the absence of eyes are exclusively found in namanereidins living in subterranean habitats. A hypothetical evolutionary derivation of bifid jaws is proposed, based upon observations of jaw morphology of several species. These exclusive troglomorphic characters (bifid jaws, eyeless) are regarded as convergent features to aphotic environments, and they should be discouraged as indicators of common ancestry. The new species, herein described as Namanereis christopheri sp. n., was collected in a cave 435 m above sea level in Saint Vincent, Caribbean Sea. The species resembles N. cavernicola but it differs because it has shorter tentacular cirri, margin of prostomium entire, rounded neuropodial lobes and broader dorsal cirri throughout body. A key to identify all known Namanereis species is included.

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Section: Methodsmentioning

confidence: 99%

The troglomorphic adaptations of Namanereidinae (Annelida, Nereididae) revisited, including a redescription of Namanereis cavernicola (Solís-Weiss & Espinasa, 1991), and a new Caribbean species of Namanereis Chamberlin, 1919

Conde-Vela¹

2017

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“…Relaxing chemicals may be used to facilitate imaging of live specimens to assure visibility of key morphological parts (e.g. proboscis eversion in polychaetes; Nygren et al 2011;Bonyadi-Naeini et al 2016). b) Microscopic organisms: light microscopy and/or electron microscopy micrographs must show all the important details of the cells or the colonies that are necessary for identification.…”

Section: Biological Specimens and Environmental Samplesmentioning

confidence: 99%

Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life

Rimet¹,

Aylagas²,

Borja³

et al. 2021

MBMG

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DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.

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“…At the end of the experiment, harvested polychaetes were immersed in 8% magnesium chloride (MgCl 2 ) in seawater for relaxation (Bonyadi- Naeini, Rahimian, & Glasby, 2016;Naeini & Rahimian, 2009). Relaxed polychaetes were easily sampled for survival and growth measurements.…”

Section: Survival Growth and Biomass Measurementsmentioning

confidence: 99%

Survival, growth and biomass of mud polychaete Marphysa iloiloensis (Annelida: Eunicidae) under different culture techniques

Mandario

2020

Aquac Res

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Polychaetes are commercially exploited as fishing bait and supplemental diet to crustacean and finfish broodstock. However, scarcity of supply and biosecurity issues limits its use. Polychaete aquaculture has become the best alternative to wild resources. In this study, two different culture techniques (1-sediment tank throughout the culture period and 2-nursery tank with bioflocs for 30 days; thereafter, juveniles were transferred to sediment tank until harvest) using two diets [bioflocs (BF) and feed mill sweepings (FS)] were tested to determine the survival, growth and biomass of polychaete Marphysa iloiloensis (Glasby et al., Zootaxa, 2019, 4674) in the grow-out.The 180-day trial was conducted in 16 tanks (0.20 m 2 ) stocked with 5,066 ± 575 M. iloiloensis trochophore larvae/tank which were assigned randomly into four culture treatments with four replicates each. The treatments were labelled as: (a) BFS -fed BF in sediment tank for 180 days; (b) BF + BFS -fed BF in nursery tank for 30 days, juveniles were transferred in sediment tank and fed BF for another 150 days; (c) FSS -fed FS in sediment tank for 180 days; and (d) BF + FSS -fed BF in nursery tank for 30 days, juveniles are transferred in sediment tank and fed FS for another 150 days. Results showed that polychaetes in BF + FSS had significantly higher survival than BFS. Polychaetes in both BF + FSS and FSS had significantly higher body weight than BF + BFS and BFS, while biomass in BF + FSS (175.73 ± 42.25 g/tank) was significantly higher compared to other treatments (p < .05). Polychaetes fed with FS had 61%-64% protein and 12%-13% fat, while those fed with BF exclusively had 66%-70% protein and 3.50%-4.50% fat. The findings indicate that FS whether or not BF is included in the diet can improve both survival and growth of polychaetes but significantly higher biomass can be achieved with the combination of BF and FS.Additionally, polychaetes in BF + FSS contained 64% protein and 13% fat. Therefore, BF can be used as an alternative diet to FS in the early life stages of M. iloiloensis and nursery rearing using BF is imperative to increase its biomass in the grow-out production. K E Y W O R D Sbiofloc, marine annelid, Marphysa iloiloensis, mudworm, polychaete culture

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A new substance to relax polychaete worms (Annelida) prior to morphological study

Cited by 8 publications

References 20 publications

The troglomorphic adaptations of Namanereidinae (Annelida, Nereididae) revisited, including a redescription of Namanereis cavernicola (Solís-Weiss & Espinasa, 1991), and a new Caribbean species of Namanereis Chamberlin, 1919

The troglomorphic adaptations of Namanereidinae (Annelida, Nereididae) revisited, including a redescription of Namanereis cavernicola (Solís-Weiss & Espinasa, 1991), and a new Caribbean species of Namanereis Chamberlin, 1919

Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life

Survival, growth and biomass of mud polychaete Marphysa iloiloensis (Annelida: Eunicidae) under different culture techniques

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A new substance to relax polychaete worms (Annelida) prior to morphological study

Cited by 8 publications

References 20 publications

The troglomorphic adaptations of Namanereidinae (Annelida, Nereididae) revisited, including a redescription of Namanereis cavernicola (Solís-Weiss &amp; Espinasa, 1991), and a new Caribbean species of Namanereis Chamberlin, 1919

The troglomorphic adaptations of Namanereidinae (Annelida, Nereididae) revisited, including a redescription of Namanereis cavernicola (Solís-Weiss &amp; Espinasa, 1991), and a new Caribbean species of Namanereis Chamberlin, 1919

Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life

Survival, growth and biomass of mud polychaete Marphysa iloiloensis (Annelida: Eunicidae) under different culture techniques

Contact Info

Product

Resources

About

The troglomorphic adaptations of Namanereidinae (Annelida, Nereididae) revisited, including a redescription of Namanereis cavernicola (Solís-Weiss & Espinasa, 1991), and a new Caribbean species of Namanereis Chamberlin, 1919

The troglomorphic adaptations of Namanereidinae (Annelida, Nereididae) revisited, including a redescription of Namanereis cavernicola (Solís-Weiss & Espinasa, 1991), and a new Caribbean species of Namanereis Chamberlin, 1919