Neostagonosporella sichuanensis gen. et sp. nov. (Phaeosphaeriaceae, Pleosporales) on Phyllostachys heteroclada (Poaceae) from Sichuan Province, China

Abstract

Neostagonosporellasichuanensis sp. nov. was found on Phyllostachysheteroclada collected from Sichuan Province in China and is introduced in a new genus Neostagonosporella gen. nov. in this paper. Evidence for the placement of the new taxon in the family Phaeosphaeriaceae is supported by morphology and phylogenetic analysis of a combined LSU, SSU, ITS and TEF 1-α DNA sequence dataset. Maximum-likelihood, maximum-parsimony and Bayesian inference phylogenetic analyses support Neostagonosporella as a distinct genus within this family. The new genus is compared with related genera of Phaeosphaeriaceae and full descriptions and illustrations are provided. Neostagonosporella is characterised by its unique suite of characters, such as multiloculate ascostromata and cylindrical to fusiform, transversely multiseptate, straight or curved ascospores, which are widest at the central cells. Conidiostromata are multiloculate, fusiform to long fusiform or rhomboid, with two types conidia; macroconidia vermiform or subcylindrical to cylindrical, transversely multiseptate, sometimes curved, almost equidistant between septa and microconidia oval, ellipsoidal or long ellipsoidal, aseptate, rounded at both ends. An updated phylogeny of the Phaeosphaeriaceae based on multigene analysis is provided.

Bambusicolous Fungi in Pleosporales: Introducing Four Novel Taxa and a New Habitat Record for Anastomitrabeculia didymospora

“…Bambusicolous fungi are highly diverse and distributed in various families within Pleosporales. Since 2015, over 85 bambusicolous species have been introduced in Pleosporales [ 3 , 27 , 33 , 39 , 41 , 45 , 46 , 49 , 50 , 52 , 55 , 59 , 63 , 68 , 71 , 74 , 76 , 77 , 78 , 79 , 80 , 100 , 104 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 ]. Even though novel taxa have been continuedly introduced in recent years, studies into the diversity of bambusicolous fungi correlating with specific bamboo genera are still limited due to the lack of host species identification.…”

“…Over the last two decades, taxonomic studies of bambusicolous fungi have become an interesting research topic for many Asian mycologists [ 3 , 12 , 13 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 ]. The taxonomic classification of bambusicolous fungi based on molecular phylogeny was initially carried out by Tanaka et al [ 12 ], who introduced a novel family Tetraplosphaeriaceae to accommodate tetraploa-like taxa in Pleosporales.…”

“…Noteworthily, major studies of bambusicolous ascomycetes with modern taxonomic treatments have been carried out by Dai et al [ 3 ], Liu et al [ 31 , 32 ], Phookamsak et al [ 39 ], and Tanaka et al [ 27 ]. Up to now, more than 175 bambusicolous ascomycetes have been described based on morphological and phylogenetic evidence [ 13 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 ]. However, there have been few thorough studies into the phylogeny-based taxonomy of bambusicolous fungi preceding 2002 [ 11 ], causing more than 80% of bambusicolous fungi to lack molecular data that could clarify their phylogenetic placement.…”

“…Many families in Pleosporales were initially introduced to accommodate bambusicolous fungi mainly such as Anastomitrabeculiaceae [ 71 ], Astrosphaeriellaceae [ 39 ], Bambusicolaceae [ 72 ], Occultibambusaceae [ 3 ], Parabambusicolaceae [ 27 ], Pseudoastrosphaeriellaceae [ 39 ], Roussoellaceae [ 32 ], Shiraiaceae [ 73 ] and Tetraploasphaeriaceae [ 12 ]. Furthermore, bambusicolous fungi are also distributed among many families in Pleosporales such as Aigialaceae, Dictyosporiaceae, Didymosphaeriaceae, Halotthiaceae, Lentitheciaceae, Ligninsphaeriaceae, Longipedicellataceae, Lophiotremataceae, Periconiaceae, Phaeosphaeriaceae, and Pleosporaceae [ 27 , 31 , 41 , 49 , 52 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 ], suggesting that bambusicolous fungi are diverse in Pleosporales. In this study, we also found the bambusicolous fungi in Pleosporales with the intention of providing a better understanding of their taxonomy placement.…”

Additions to Phaeosphaeriaceae (Pleosporales): Elongaticollum gen. nov., Ophiosphaerella taiwanensis sp. nov., Phaeosphaeriopsis beaucarneae sp. nov. and a new host record of Neosetophoma poaceicola from Musaceae

“…Newly generated sequence data were initially subjected to blast search in NCBI to obtain the closest matches in GenBank. Sequences generated from this study were analyzed with related taxa in the family Phaeosphaeriaceae, which were obtained from GenBank and from recently published data (Bakhshi et al 2019;Hyde et al 2019;Maharachchikumbura et al 2019;Yang et al 2019;Mapook et al 2020) (Table 1). The combined dataset consisted of 168 sequences including our newly generated sequences.…”