New species of Hohenbuehelia, with comments on the Hohenbuehelia atrocoerulea – Nematoctonus robustus species complex

Abstract

Four new species of Hohenbuehelia (Fungi: Pleurotaceae) are described in the group of Hohenbuehelia atrocoerulea and Hohenbuehelia grisea. Hohenbuehelia algonquinensis, found on Pinus in Ontario, Canada, may be distinguished macroscopically from bluish collections of H. atrocoerulea and watery grey-brown collections of H. grisea by its coal-black pileus. Hohenbuehelia canadensis, on or associated with Pinus in both Ontario and Alberta, Canada, and Hohenbuehelia nimueae, on Salix in Ontario and Abies in Wyoming, USA, have similarly dark fruiting bodies and were previously misidentified as H. approximans (which we treat as a synonym of H. grisea), H. atrocoerulea, H. mustialensis or H. nigra. The latter species is shown to be a member of Resupinatus, despite the presence of prominent metuloid cystidia in its hymenium. Hohenbuehelia carlothornii has been found in Costa Rica; collections of the sexual fruiting bodies were previously identified as H. grisea and isolates from soil nematodes were identified by the anamorph name Nematoctonus robustus. That name has been treated as a synonym of H. atrocoerulea but, given the genetic and geographic variation within this complex, we transfer it to Hohenbuehelia as a distinct species. Sequences of the nuclear ribosomal DNA internal transcribed spacer region (ITS), the D1/D2 variable region of the large subunit gene, and a portion of the translation elongation factor (TEF1) gene provide good separation and support for these new species. A key to the dimidiate species of Hohenbuehelia of North America and Europe is provided.

Additions to Hohenbuehelia (Basidiomycota, Pleurotaceae): two new species and notes on H. tristis from northern Thailand

“…), smooth pileus surface, larger basidiospores (7.2–10.4 × 4.5–6.1 µm), larger pleurocystidia (56–103 × 11–19 µm), and an ixotrichoderm or ixocutis pileipellis ( Ainsworth et al 2016 ). Hohenbuehelia carlothornii described from Costa Rica, is different by having off-white basidiomata with a large pseudostipe (20–32 × 14–25 mm), presence of cheilocystidia ( Consiglio et al 2018b ). Hohenbuehelia concentrica from Singapore, has larger basidiomata (80 mm wide), larger basidiospores (8–8.5 × 6–6.7 µm), and absence of cheilocystidia ( Corner 1994 ).…”

“…(voucher RGT 870601/12 UWO) with 3.5% (21/599) differences in the ITS sequence, 1.4% (12/839) in the LSU sequence, and 6.66% (35/540) in the tef 1 sequence. Moreover, the morphologies of both species are completely different, with H. algonquinensis having glossy black pileus, ungulate to dimidiate basidiomata and contrasting white or off-white lamellae ( Consiglio et al 2018b ).…”

“… Hohenbuehelia bonii differs by having a smooth pileus surface and larger pleurocystidia (56–103 × 11–19 µm) ( Ainsworth et al 2016 ). Hohenbuehelia carlothornii differs by having finely white-tomentose pileus, smaller basidiospores (7–8.2 × 3.1–3.7 µm), smaller basidia (19–24 × 5.2–6.8 µm), smaller cheilocystidia (13–17 × 4.9–9.4 µm), and larger pleurocystidia (55–63 × 14–18 µm) ( Consiglio et al 2018b ). Hohenbuehelia concentrica has larger basidiomata (80 mm wide), ovoid basidiospores (8–8.5 × 6–6.7 µm), and no cheilocystidia ( Corner 1994 ).…”

Morphological characteristics and phylogenetic analyses revealed four new species of Agaricales from China

“…Morphologically, H. longipes mainly differs from H. tomentosa in having a longer stipe (40-60 (80) × 3-5 (8) mm in H. longipes, 20-40 × 7-9 mm in H. tomentosa), a brown-ochraceous to isabelline pileus, and whitish-ochraceous gills (Thorn and Barron, 1986;Watling and Gregory, 1989). Hohenbuehelia petaloides, H. angustata, H. wilhelmii, and H. tremula all lack a subcentral, fibrous striate stipe (Singer and Kuthán, 1980;Thorn and Barron, 1986;Corner, 1994;Consiglio et al, 2018). Hohenbuehelia thornii has a gelatine layer in its pileipellis, which is absent in H. tomentosa (Consiglio, 2017).…”

The macrofungi of Gozo (Maltese Islands): an annotated checklist

“…Moreover, the teleomorphic phase of this genus is characterised by wood-dwelling, fleshy fungi with a small, pleurotoid stem-less fruiting bodies covered with a gelatinous cuticle. The material from Gozo was identified as Hohenbuehelia cyphelliformis using the works of Consiglio & al. (2018) and Krisai-Greilhuber (1994).…”