Novel genera and species of coniothyrium-like fungi in <i>Montagnulaceae</i> (<i>Ascomycota</i>)

Verkley, G.J.M.; Dukik, Karolina; Renfurm, R.; Göker, Markus; Stielow, J. Benjamin

doi:10.3767/003158514x679191

Cited by 110 publications

(95 citation statements)

References 49 publications

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“…Taxonomy of this group coniothyrium-like fungi is quite complex and subjected to periodical updates, mainly to accommodate the wealth of DNA sequence data and phylogenetic reassessments of ex-type and other reference strains preserved in culture collections (Verkley et al, 2014;Ariyawansa et al, 2015;Chen et al, 2015). On the other hand, the ITS marker is generally reliable for strain typing and identifications to genus level among this group of fungi (Verkley et al, 2014). Species of Phoma-like fungi are common endophytes of grasses, such as the temperate species Dactylis glomerata and Holcus lanatus (Sánchez Márquez et al, 2007.…”

Section: Resultsmentioning

confidence: 99%

Endophytic fungi from Brachiaria grasses in Brazil and preliminary screening of Sclerotinia sclerotiorum antagonists

Gama

Santos

Abreu

et al. 2020

Sci. agric. (Piracicaba, Braz.)

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Fungal endophytes of Brachiaria, a nonhost of Sclerotinia sclerotiorum, may harbor species with antagonistic effects against this plant pathogen. The objective of this work was to investigate the diversity of endophytic fungi associated with different Brachiaria species and hybrids and evaluate their potential to inhibit the plant pathogen S. sclerotiorum. Stem samples from 39 Brachiaria spp. plants were collected in pasture fields and experimental areas of three states of Brazil resulting in 74 endophytes isolated. Twenty-eight species were identified by sequences of the Internal Transcribed Spacer (ITS) and 18S rDNA regions. Paraconiothyrium sp. was the most abundant endophyte, accounting for 24 % (14 isolates) of total, and it was isolated from B. ruziziensis, B. decumbens, B. humidicola, and B. brizantha. Phoma sorghina was the second most abundant taxon, followed by Sarocladium strictum, and Plenodomus sp. In vitro analyses showed that Paraconiothyrium sp., Sarocladium kiliense, Acremonium curvulum, Setophoma terrestris, Dissoconium sp., and Cladosporium flabelliforme exhibited antagonistic activity against S. sclerotiorum, with percentages of growth inhibition ranging from 25 to 60 (p < 0.05). Paraconiothyrium sp. BBXE1 (60 %), BBPB4.1 (60 %), BCMT4.1 (54 %), and S. kiliense (54 %) showed the highest values of Antagonism Percentages (AP). Therefore, fungi with inhibitory activity against S. sclerotiorum such as Paraconiothyrium sp. are naturally endophytic in Brachiaria grasses. Sampling Brachiaria plants Thirty-nine disease-free plants of different species and hybrids of Brachiaria were sampled: B. ruziziensis; B. decumbens cv. Basilisk; B. mutica cv. Angola; hybrid cv. Mulato I and cv. Mulato II; B. humidicola common, cv. Llanero and cv. Tupi; B. brizantha cv. Piatã, cv. Xaraés, and cv. Marandu, in which 11 were collected from pasture fields and 28 from experimental plots. The samples were collected during the rainy and dry seasons from

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Section: Resultsmentioning

confidence: 99%

Endophytic fungi from Brachiaria grasses in Brazil and preliminary screening of Sclerotinia sclerotiorum antagonists

Gama

Santos

Abreu

et al. 2020

Sci. agric. (Piracicaba, Braz.)

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“…For example, the observed shapes of the conidiophores and conidia were also observed in pycnidia of species belonging to other genera. DNA sequencing facilitated numerous systematic revisions, and resulted in the addition of new genera to the long-standing coelomycetes such as Septoria , Mycosphaerella , Phoma , Coniothyrium and Phomopsis ( Diaporthe ) (Crous et al., 2001, de Gruyter et al., 2013, Gomes et al., 2013, Verkley et al., 2013, Verkley et al., 2014). Therefore, a comprehensive study was needed to place them with high confidence in the context of a phylogenetically-based classification.…”

Section: Discussionmentioning

confidence: 99%

Wood staining fungi revealed taxonomic novelties in Pezizomycotina: New order Superstratomycetales and new species Cyanodermella oleoligni

Nieuwenhuijzen

Houbraken

Adan

et al. 2016

Studies in Mycology

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A culture-based survey of staining fungi on oil-treated timber after outdoor exposure in Australia and the Netherlands uncovered new taxa in Pezizomycotina. Their taxonomic novelty was confirmed by phylogenetic analyses of multi-locus sequences (ITS, nrSSU, nrLSU, mitSSU, RPB1, RPB2, and EF-1α) using multiple reference data sets. These previously unknown taxa are recognised as part of a new order (Superstratomycetales) potentially closely related to Trypetheliales (Dothideomycetes), and as a new species of Cyanodermella, C. oleoligni in Stictidaceae (Ostropales) part of the mostly lichenised class Lecanoromycetes. Within Superstratomycetales a single genus named Superstratomyces with three putative species: S. flavomucosus, S. atroviridis, and S. albomucosus are formally described. Monophyly of each circumscribed Superstratomyces species was highly supported and the intraspecific genetic variation was substantially lower than interspecific differences detected among species based on the ITS, nrLSU, and EF-1α loci. Ribosomal loci for all members of Superstratomyces were noticeably different from all fungal sequences available in GenBank. All strains from this genus grow slowly in culture, have darkly pigmented mycelia and produce pycnidia. The strains of C. oleoligni form green colonies with slimy masses and develop green pycnidia on oatmeal agar. These new taxa could not be classified reliably at the class and lower taxonomic ranks by sequencing from the substrate directly or based solely on culture-dependent morphological investigations. Coupling phenotypic observations with multi-locus sequencing of fungi isolated in culture enabled these taxonomic discoveries. Outdoor situated timber provides a great potential for culturable undescribed fungal taxa, including higher rank lineages as revealed by this study, and therefore, should be further explored.

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“…A BLAST search of partial sequences of large subunit and internal transcribed spacer region (ITS) revealed that TR-022 possessed the highest sequence similarities with Paraconiothyrium brasiliense CBS 122851 (99.7%) and Paraphaeosphaeria sporulosa CBS 824.68 (96.8%), respectively. The phylogenetic tree based on ITS sequences clearly indicated that TR-022 was nested in a ParaphaeosphaeriaParaconiothyrium clade 11 and did not cluster with any species within a clade ( Figure 2). Thus, TR-022 was designated as a Paraphaeosphaeria sp.…”

Section: Taxonomy Of the Producing Strain Tr-022mentioning

confidence: 96%

“…The ITS sequence of TR-022 was aligned with those of 24 fungal strains selected based on the BLAST result as well as on taxonomical references. 11,20 The phylogenetic tree was constructed by MEGA ver. 6.0.…”

Section: Taxonomic Studymentioning

confidence: 99%

Paraphaeosphaeride D and berkleasmin F, new circumventors of arbekacin resistance in MRSA, produced by Paraphaeosphaeria sp. TR-022

et al. 2016

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Two new compounds, designated paraphaeosphaeride D (1) and berkleasmin F (2) together with a previously known compound, berkleasmin A (3), isolated from a culture broth of the fungus Paraphaeosphaeria sp. TR-022, proved to be new circumventors of arbekacin (ABK) resistance in methicillin-resistant Staphylococcus aureus (MRSA). The structures of 1 and 2 were elucidated by spectroscopic analyses, including various NMR experiments. All compounds showed 10-100 times ABK circumvention activities using the paper disc method and reduced the MIC values of ABK against MRSA from 16 μg ml(-1) to 4 μg ml(-1) (fourfold) using the agar dilution method. These new compounds might be promising lead compounds for developing circumventors of ABK resistance in MRSA.

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Novel genera and species of coniothyrium-like fungi in Montagnulaceae (Ascomycota)

Cited by 110 publications

References 49 publications

Endophytic fungi from Brachiaria grasses in Brazil and preliminary screening of Sclerotinia sclerotiorum antagonists

Endophytic fungi from Brachiaria grasses in Brazil and preliminary screening of Sclerotinia sclerotiorum antagonists

Wood staining fungi revealed taxonomic novelties in Pezizomycotina: New order Superstratomycetales and new species Cyanodermella oleoligni

Paraphaeosphaeride D and berkleasmin F, new circumventors of arbekacin resistance in MRSA, produced by Paraphaeosphaeria sp. TR-022

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