Sarcophaga (Liosarcophaga) dux (Diptera: Sarcophagidae): A flesh fly species of medical importance

Sukontason, Kabkaew L.; Sanit, Sangob; Klong-klaew, Tunwadee; Tomberlin, Jeffery K.; Sukontason, Kom

doi:10.1186/0717-6287-47-14

Cited by 25 publications

(8 citation statements)

References 30 publications

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“…The influence of rearing temperature and Intrapuparial age on the expression of DEGs was tested by a two-way ANOVA analysis (Supplementary Material 2), the result showed that the interaction of age×temperature has a significant influence on the expression profiles of S. dux Intra-puparial DEGs. [34]. In addition, the developmental durations of this species from the larvae to pupariation were 72-96h in ranging between 27.1-29.8°C in Thailand [52], 168h at 29.5°C in Guam [53], 196-244h at 25°C in South Africa [54], and 114, 108, 86h at 25, 28, 31°C in our study.…”

Section: Overall and Detailed Expression Profiles Of The Degsupporting

confidence: 51%

“…Sarcophaga (Liosarcophaga) dux Thomson, 1869 (Diptera: Sarcophagidae), a synanthropic flesh fly species in many parts of the world, is of medical importance as a vector of disease or parasitic, myiasis-producing agent [34], and as forensically important species with the necrophagous habits, it was found to colonize corpses of a male infant in Malaysia [35]. This species widely distributed around the world, such as Oriental region.…”

Section: Introductionmentioning

confidence: 99%

“…This species widely distributed around the world, such as Oriental region. Previous entomology investigations on S. dux have focused on the description of important morphological features in each developmental stage [34], molecular identification analysis by mitochondrial cytochrome oxidase gene subunits I and II (COI and COII) sequences [36], the human death investigations in forensic entomology [34]. However, there are still lacking a detailed investigation of S. dux development.…”

Section: Introductionmentioning

confidence: 99%

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Development of Sarcophaga dux (Diptera: Sarcophagidae) at constant temperatures and differential gene expression for age estimation of intrapuparial

Zhang

Shang

et al. 2019

Preprint

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BackgroundSarcophaga dux (Diptera: Sarcophagidae) is a necrophagous flesh fly with potential forensic value in estimating minimum postmortem interval (PMImin). The basic developmental data and precise intrapuparial age estimates are significant for PMImin estimationof application entomological data in legal medicine.MethodsThe development parameters of S. dux at seven constant temperatures from 16°C to 34°C were investigated by rearing using pig lung in the artificial climate box. The appropriate reference genes and intrapuparial differentially expressed genes (DEGs) of S. dux at constant temperatures 34, 25 and 16°C were selected and analyzed using RT-qPCR for more precisely age estimations. ResultsThe developmental durations of S. dux at 16, 19, 22, 25, 28, 31 and 34°C from larviposition to adult eclosion were 1478.6±18.3, 726.1±15.8, 538.5±0.9, 394.1±9.5, 375.6±10.8, 284.1±7.3, and 252.5±6.1 h, respectively. The thermal summation constant of S. dux was 5341.71±249.29 degree hours, and the developmental threshold temperature was 12.266±0.35°C. The most reliable reference genes under intrapuparial and different temperature in our study were: GST1 and 18S rRNA for 34°C group, GST1 and RPL49 for 25 °C group, and 18S rRNA and 28S rRNA for 16 °C group，the four differential expression genes (Hsp60, A-alpha, ARP, RPL8) can be used to more precisely intrapuparial age estimation of S. dux. ConclusionsThe basic developmental data of S. dux at constant temperatures, such as body length changing of the larva period, accumulated degree hours and duration of development, to establish developmental models that can be used to estimate the PMImin. The selection and evaluation of appropriate reference genes at different experimental conditions are primary with RT-qPCR. The differentially expressed gene can contribute to more accuracy age estimations of S. dux intrapuparial. The result from this study can make contributes to the use of S.dux for estimating PMImin.

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Section: Overall and Detailed Expression Profiles Of The Degsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of Sarcophaga dux (Diptera: Sarcophagidae) at constant temperatures and differential gene expression for age estimation of intrapuparial

Zhang

Shang

et al. 2019

Preprint

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“…Morphological characteristics of immature and adult stages among flesh fly species are very similar, thus making identification difficult, particularly for non-expert taxonomists [13]. Since the developmental times of flesh flies are species specific, the correct identification at the species level is a primary step for estimating the PMI min [14,15]. Therefore, a potential tool is needed which can discriminate the species regardless of life-history stage is needed [16].…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand

Samerjai

Sukontason

Sontigun

et al. 2019

Insects

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Flesh flies (Sarcophagidae) are necrophagous insects initially colonizing on a corpse. The species-specific developmental data of the flies collected from a death scene can be used to estimate the minimum postmortem interval (PMImin). Thus, the first crucial step is to correctly identify the fly species. Because of the high similarity among species of flesh flies, DNA-based identification is considered more favorable than morphology-based identification. In this study, we demonstrated the effectiveness of combined sequences (2216 to 2218 bp) of cytochrome c oxidase subunit I and II genes (COI and COII) for identification of the following 14 forensically important flesh fly species in Thailand: Boettcherisca nathani Lopes, Fengia ostindicae (Senior-White), Harpagophalla kempi (Senior-White), Liopygia ruficornis (Fabricius), Lioproctia pattoni (Senior-White), Lioproctia saprianovae (Pape & Bänziger), Parasarcophaga albiceps (Meigen), Parasarcophaga brevicornis (Ho), Parasarcophaga dux (Thomson), Parasarcophaga misera (Walker), Sarcorohdendorfia antilope (Böttcher), Sarcorohdendorfia inextricata (Walker), Sarcorohdendorfia seniorwhitei (Ho) and Seniorwhitea princeps (Wiedemann). Nucleotide variations of Thai flesh flies were evenly distributed throughout the COI-COII genes. Mean intra- and interspecific variations ranged from 0.00 to 0.96% and 5.22% to 12.31%, respectively. Using Best Match (BM) and Best Close Match (BCM) criteria, identification success for the combined genes was 100%, while the All Species Barcodes (ASB) criterion showed 76.74% success. Maximum Likelihood (ML) and Bayesian Inference (BI) phylogenetic analyses yielded similar tree topologies of monophyletic clades between species with very strong support values. The achieved sequences covering 14 forensically important flesh fly species including newly submitted sequences for B. nathani, F. ostindicae and S. seniorwhitei, can serve as a reliable reference database for further forensic entomological research in Thailand and in other areas where those species occur.

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“…Depending on the behavior, there are facultative and obligatory myiases. Frequently reported cases are from tropical countries and the most common infestation is by Cordylobia anthropophaga from Africa or Dermatobia hominis from South America [5] [8]. Based on an elegant review by Sueyoshi [9] in 2015, a total of 209 cases of facultative myiases have been reported in Japan.…”

Section: Introductionmentioning

confidence: 99%

A Case Report of Intestinal Myiasis in a Japanese Man

Watanabe¹,

Kato²,

Ichiyanagi³

et al. 2016

OJPathology

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We recently experienced a case of intestinal myiasis caused by maggots of fly, Sarcophagidae. A 61-year-old Japanese man presented in August 2015 with a 2-day history of diarrhea and white maggots in his stool. Two days before his presentation, he took Chinese noodles and lettuce. At his presentation, we observed a number of discharged insect bodies in his stool. The insect bodies were identified as the larvae of the 3 rd instar flesh fly Sarcophagidae based on macroscopic and microscopic examinations. We finally diagnosed as intestinal myiasis due to the fact that the patient had the insect bodies mixed in his diet, lettuce. A few days after the treatment with antibiotics, his symptoms disappeared. Accurate diagnosis of intestinal myiasis in developed countries is necessary to avoid ineffective treatment.

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Sarcophaga (Liosarcophaga) dux (Diptera: Sarcophagidae): A flesh fly species of medical importance

Cited by 25 publications

References 30 publications

Development of Sarcophaga dux (Diptera: Sarcophagidae) at constant temperatures and differential gene expression for age estimation of intrapuparial

Development of Sarcophaga dux (Diptera: Sarcophagidae) at constant temperatures and differential gene expression for age estimation of intrapuparial

Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand

A Case Report of Intestinal Myiasis in a Japanese Man

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