Semicontinuous and Continuous Production of Chloroperoxidase by Caldariomyces fumago Immobilized in k -Carrageenan

Abstract

Three strains of Caldariomycesfumago were immobilized in 4% k-carrageenan and tested for semicontinuous production of chloroperoxidase (CPO). Over an 80-day period, growing in defined medium, C. fumago strains

Continuous and Batch Production of Chloroperoxidase by Mycelial Pellets of Caldariomyces fumago in an Airlift Fermentor

“…Semicontinuous production of CPO by K-carrageenanimmobilized C. fumago has been demonstrated by using shake flasks (3). In that study (3), CPO-producing cultures were maintained over 4 months with medium replacement, until the beaded cultures had grown so much that shaking caused continual shedding of mycelial fragments.…”

“…Semicontinuous production of CPO by K-carrageenanimmobilized C. fumago has been demonstrated by using shake flasks (3). In that study (3), CPO-producing cultures were maintained over 4 months with medium replacement, until the beaded cultures had grown so much that shaking caused continual shedding of mycelial fragments. Batch airlift fermentor cultures also produced high levels of CPO under semicontinuous conditions; a single batch of K-carrageenan-immobilized hyphae was used in four consecutive fermentor cultures over a period of 2 months, with there being little shedding and no sign of decreasing ability to synthesize CPO.…”

“…The fungal pellets used in this study were not resting cells; enzyme production was growth related. In shake flasks and batch fermentors, CPO was produced early on in the culture but reached a maximum level near the end of the increase in biomass (3,18). In continuously fed fermentor cultures, CPO eventually reached a constant concentration in the effluent.…”

“…pH and enzyme, sugar, nitrate, and pigment concentrations were determined for the supernatant samples. Enzyme activity was determined kinetically by measuring the chlorinating activity of CPO, as described previously (18), and is expressed as milligrams liter-' (3). Pigment levels were monitored at 575 nm by using potassium phosphate-H3PO4 buffer (20 mM, pH 5.5) as diluent and blank.…”

“…Chloroperoxidase (CPO) is an extracellular enzyme from the sooty mould Caldariomycesfumago which can catalyze the formation of various halides and halohydrins (8) and which has a potential for the removal of phenolic compounds from wastewater (1,2). The enzyme has been produced by free hyphae in a shake flask batch culture (10, 18) or a roller bottle culture (19) and by hyphae immobilized in K-carrageenan by using a semicontinuous shake flask culture (3). Since the use of mycelial pellets has been shown to offer several advantages, including improved mixing characteristics and easier downstream processing (9), and the reuse of productive mycelium has shown promise in semicontinuous shake flask culture (3), we decided to investigate continuous CPO production using mycelial pellets in an airlift fermentor.…”