Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana

Brigneti, Gianinna

doi:10.1093/emboj/17.22.6739

Cited by 975 publications

(735 citation statements)

References 39 publications

Supporting

Mentioning

691

Contrasting

Unclassified

Order By: Relevance

“…However, no substitutions were observed in 5 0 UTR and IR regions. No change was found in the core promoter region within IR region which explains the extreme conservation of this region essentially required for the virus replication as suggested earlier [3]. Five unique amino acid substitutions in C-terminal region at positions 149 (S/P), 150(A/V), 151(M/L), 156 (T/A) and 158 (T/S) of CP of the virus isolate under study were found.…”

supporting

confidence: 66%

See 1 more Smart Citation

Biological and Molecular Characterization of Cucumber mosaic virus Isolate Causing Shoestring Disease of Tomato in India which has Closer Affinity to European or East Asian Isolates of CMV

et al. 2012

View full text Add to dashboard Cite

supporting

confidence: 66%

“…protein, involves in the suppression of gene silencing [3]. RNA3 encodes 3a protein, essential for the virus movement, and the coat protein (CP), which is expressed from subgenomic RNA4.…”

mentioning

confidence: 99%

Biological and Molecular Characterization of Cucumber mosaic virus Isolate Causing Shoestring Disease of Tomato in India which has Closer Affinity to European or East Asian Isolates of CMV

et al. 2012

View full text Add to dashboard Cite

“…Cucumber mosaic virus protein 2b (CMV2b) is one of the first suppressors identified that could suppress host RNA silencing (Brigneti et al, 1998). Previously, we reported that CMV2b from the FNY strain interacted directly with Arabidopsis Argonaute1 (AGO1) in vitro and in vivo to inhibit RISC-mediated messenger RNA cleavage (Zhang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Structural basis for RNA‐silencing suppression by Tomato aspermy virus protein 2b

et al. 2008

View full text Add to dashboard Cite

The 2b proteins encoded by cucumovirus act as post-transcriptional gene silencing suppressors to counter host defence during infection. Here we report the crystal structure of Tomato aspermy virus 2b (TAV2b) protein bound to a 19 bp small interfering RNA (siRNA) duplex. TAV2b adopts an all a-helix structure and forms a homodimer to measure siRNA duplex in a length-preference mode. TAV2b has a pair of hook-like structures to recognize simultaneously two a-helical turns of A-form RNA duplex by fitting its a-helix backbone into two adjacent major grooves of siRNA duplex. The conserved p-stackings between tryptophan and the 5 0 -terminal base of siRNA duplex from both ends enhance the recognition. TAV2b further oligomerizes to form a dimer of dimers through the conserved leucine-zipper-like motif at its amino-terminal a-helix. Biochemical experiments suggest that TAV2b might interfere with the post-transcriptional gene silencing pathway by directly binding to siRNA duplex.

show abstract

“…Most plant viral genes seem to contribute to pathogenicity (Brigneti et al, 1998;Takeshita et al, 2001). Consequently, recombination with a viral transgene could potentially lead to increased virulence.…”

Section: Hgt From Gm Plants To Virusesmentioning

confidence: 99%

Risks from GMOs due to Horizontal Gene Transfer

Keese¹

2008

Environ. Biosafety Res.

131

View full text Add to dashboard Cite

Horizontal gene transfer (HGT) is the stable transfer of genetic material from one organism to another without reproduction or human intervention. Transfer occurs by the passage of donor genetic material across cellular boundaries, followed by heritable incorporation to the genome of the recipient organism. In addition to conjugation, transformation and transduction, other diverse mechanisms of DNA and RNA uptake occur in nature. The genome of almost every organism reveals the footprint of many ancient HGT events. Most commonly, HGT involves the transmission of genes on viruses or mobile genetic elements. HGT first became an issue of public concern in the 1970s through the natural spread of antibiotic resistance genes amongst pathogenic bacteria, and more recently with commercial production of genetically modified (GM) crops. However, the frequency of HGT from plants to other eukaryotes or prokaryotes is extremely low. The frequency of HGT to viruses is potentially greater, but is restricted by stringent selection pressures. In most cases the occurrence of HGT from GM crops to other organisms is expected to be lower than background rates. Therefore, HGT from GM plants poses negligible risks to human health or the environment.

show abstract

Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana

Cited by 975 publications

References 39 publications

Biological and Molecular Characterization of Cucumber mosaic virus Isolate Causing Shoestring Disease of Tomato in India which has Closer Affinity to European or East Asian Isolates of CMV

Biological and Molecular Characterization of Cucumber mosaic virus Isolate Causing Shoestring Disease of Tomato in India which has Closer Affinity to European or East Asian Isolates of CMV

Structural basis for RNA‐silencing suppression by Tomato aspermy virus protein 2b

Risks from GMOs due to Horizontal Gene Transfer

Contact Info

Product

Resources

About